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Yellow Fever Virus: Methods and Protocols [Kõva köide]

  • Formaat: Hardback, 268 pages, kõrgus x laius: 254x178 mm, 38 Illustrations, color; 49 Illustrations, black and white; XII, 268 p. 87 illus., 38 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2913
  • Ilmumisaeg: 19-Apr-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071644572
  • ISBN-13: 9781071644577
Teised raamatud teemal:
  • Kõva köide
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  • Formaat: Hardback, 268 pages, kõrgus x laius: 254x178 mm, 38 Illustrations, color; 49 Illustrations, black and white; XII, 268 p. 87 illus., 38 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2913
  • Ilmumisaeg: 19-Apr-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071644572
  • ISBN-13: 9781071644577
Teised raamatud teemal:
This volume covers a range of fundamental and advanced techniques and methods used by researchers to study the control of yellow fever. The chapters in this book cover a broad range of topics including epidemiological studies that track the spread of disease and sophisticated laboratory techniques that investigate the diseases pathogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls.





Cutting-edge and comprehensive, Yellow Fever Virus: Methods and Protocols is a valuable guide for students, researchers, and health professionals who are looking to understand and combat yellow fever. This book will inspire new discoveries, collaborations, and initiatives that will bring humanity closer to eradicating this disease.
Flow Cytometry as Immunoassay Tool for Research on Yellow Fever Virus.-
Clinical Features of Human Infection by Yellow Fever.- ELISA for Detection of
Total IgG against YF 17DD.- A Fast RT-qPCR One-Step to Quantify Yellow Fever
Virus.- Immunoenzyme Test for Detection of IgG Anti-YFV Class Antibodies
IgG-ELISA.- Immunofluorescence Assay.- Type I Interferon-Induced Modification
of Yellow Fever Virus NS5 Enhances Binding to Human STAT2.- Neutralization
Tests for Quantification of Specific Antibodies Against Yellow Fever Virus.-
Purification of Yellow Fever Virus by Liquid Chromatography.- Conventional
RT-PCR for Yellow Fever Virus.- Sequencing of Yellow Fever Virus.- Detection
of Viral Particles in Paraffinized Tissued and Cell Culture by Indirect
Immunofluorescence.- Yellow Fever Virus Genotyping Tool Using RT-qPCR
Followed by Nucleotide Sequencing and Phylogenetic Analysis.- Detection of
Viral RNA in Paraffinized Tissues by Nested RT-PCR.- Automation on the Plate
Analysis from Yellow Fever Serum Neutralization Assays.- NS5 Region Cloning
Protocol, For the Construction of an Accurate Standard Curve in the Detection
and Diagnosis of the Yellow Fever Virus.- Micro-Focus Reduction
Neutralization Tests (mFRNT) for Neutralizing Antibody (Nab) Quantification
against Yellow Fever Virus.- Quantification of Yellow Fever Virus by Plaque
Assay and Focus Formation Assay.- Thawing, Growth, and Maintenance of Vero
Cell Lines.- Yellow Fever Virus Bank Production in Stationary Cell Culture.-
Yellow Fever Virus Production in Bioreactor.- Immunoenzyme Test for Capture
of IgM Anti-YFV Class Antibodies MAC-ELISA.- Enhancing Biosafety Protocols
in Laboratories Conducting Research on the Yellow Fever Virus.- Epidemic
Outbreaks Related to Yellow Fever Viruses.