The premier comprehensive reference on biomedical optics for practitioners and students
Biophotonics is a rapidly growing field with applications in medicine, genetics, biology, agriculture, and environmental science. Written by respected experts, Biomedical Optics: Principles and Imaging is the first thorough reference and textbook on the subject. It covers:
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The fundamentals of photon transport in biological tissues, including explanations of Rayleigh and Mie scattering, Monte Carlo simulations, the radiative transport equation, and more
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Various optical imaging techniques for biological tissues, including ballistic or quasi-ballistic imaging (such as confocal microscopy, two-photon microscopy, and optical coherence tomography), diffuse imaging (such as DC, time-domain techniques, and frequency-domain techniques), and ultrasound-aided hybrid imaging (including photoacoustic tomography and ultrasound-modulated optical tomography)
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The basic physics and engineering of each imaging modality
Complete with equation derivations, examples, and case studies plus a constantly updated Web site featuring an established Monte Carlo program, other sample programs, tables and figures, and more, this is a great reference for practitioners. With an instructor's solutions manual and problems for students to complete, it's an excellent textbook for upper-level undergraduates or graduate students.
Preface.
1. INTRODUCTION. 1.1.Motivation for optical imaging.
1.2.General behavior of light in biological tissue. 1.3.Basic physics of
light-matter interaction. 1.4.Absorption and its biological origins.
1.5.Scattering and its biological origins. 1.6.Polarization and its
biological origins. 1.7.Fluorescence and its biological origins. 1.8.Image
characterization. 1.9.References. 1.10.Further readings. 1.11.Problems.
2.
RAYLEIGH THEORY AND MIE THEORY FOR A SINGLE SCATTERER. 2.1.Introduction.
2.2.Summary of the Rayleigh theory. 2.3.Numerical example of the Rayleigh
theory. 2.4.Summary of the Mie theory. 2.5.Numerical example of the Mie
theory. 2.6.Appendix 2.A. Derivation of the Rayleigh theory. 2.7.Appendix
2.B. Derivation of the Mie theory. 2.8.References. 2.9.Further readings.
2.10.Problems.
3. MONTE CARLO MODELING OF PHOTON TRANSPORT IN BIOLOGICAL
TISSUE. 3.1.Introduction. 3.2.Monte Carlo method. 3.3.Definition of problem.
3.4.Propagation of photons. 3.5.Physical quantities. 3.6.Computational
examples. 3.7.Appendix 3.A. Summary of MCML. 3.8.Appendix 3.B. Probability
density function. 3.9.References. 3.10.Further readings. 3.11.Problems.
4.
CONVOLUTION FOR BROADBEAM RESPONSES. 4.1.Introduction. 4.2.General
formulation of convolution. 4.3.Convolution over a Gaussian beam.
4.4.Convolution over a top-hat beam. 4.5.Numerical solution to convolution.
4.6.Computational examples. 4.7.Appendix 4.A. Summary of CONV.
4.8.References. 4.9.Further readings. 4.10.Problems.
5. RADIATIVE TRANSFER
EQUATION AND DIFFUSION THEORY. 5.1.Introduction. 5.2.Definitions of physical
quantities. 5.3.Derivation of the radiative transport equation. 5.4.Diffusion
theory. 5.5.Boundary conditions. 5.6.Diffuse reflectance. 5.7.Photon
propagation regimes. 5.8.References. 5.9.Further readings. 5.10.Problems.
6.
HYBRID MODEL OF MONTE CARLO METHOD AND DIFFUSION THEORY. 6.1.Introduction.
6.2.Definition of problem. 6.3.Diffusion theory. 6.4.Hybrid model.
6.5.Numerical computation. 6.6.Computational examples. 6.7.References.
6.8.Further readings. 6.9.Problems.
7. SENSING OF OPTICAL PROPERTIES AND
SPECTROSCOPY. 7.1.Introduction. 7.2.Collimated transmission method.
7.3.Spectrophotometry. 7.4.Oblique-incidence reflectometry. 7.5.White-light
spectroscopy. 7.6.Time-resolved measurement. 7.7.Fluorescence spectroscopy.
7.8.Fluorescence modeling. 7.9.References. 7.10.Further readings.
7.11.Problems.
8. BALLISTIC IMAGING AND MICROSCOPY. 8.1.Introduction.
8.2.Characteristics of ballistic light. 8.3.Time-gated imaging.
8.4.Spatial-frequency filtered imaging. 8.5.Polarization-difference imaging.
8.6.Coherence-gated holographic imaging. 8.7.Optical heterodyne imaging.
8.8.Radon transformation and computed tomography. 8.9.Confocal microscopy.
8.10.Two-photon microscopy. 8.11.Appendix 8.A. Holography. 8.12.References.
8.13.Further readings. 8.14.Problems.
9. OPTICAL COHERENCE TOMOGRAPHY.
9.1.Introduction. 9.2.Michelson interferometry. 9.3.Coherence length and
coherence time. 9.4.Time-domain OCT. 9.5.Fourier-domain rapid scanning
optical delay line. 9.6.Fourier-domain OCT. 9.7.Doppler OCT. 9.8.Group
velocity dispersion. 9.9.Monte Carlo modeling of OCT. 9.10.References.
9.11.Further readings. 9.12.Problems.
10. MUELLER OPTICAL COHERENCE
TOMOGRAPHY. 10.1.Introduction. 10.2.Mueller calculus versus Jones calculus.
10.3.Polarization state. 10.4.Stokes vector. 10.5.Mueller matrix.
10.6.Mueller matrices for a rotator, a polarizer, and a retarder.
10.7.Measurement of Mueller matrix. 10.8.Jones vector. 10.9.Jones matrix.
10.10.Jones matrices for a rotator, a polarizer, and a retarder.
10.11.Eigenvectors and eigenvalues of Jones matrix. 10.12.Conversion from
Jones calculus to Mueller calculus. 10.13.Degree of polarization in OCT.
10.14.Serial Mueller OCT. 10.15.Parallel Mueller OCT. 10.16.References.
10.17.Further readings. 10.18.Problems.
11. DIFFUSE OPTICAL TOMOGRAPHY.
11.1.Introduction. 11.2.Modes of diffuse optical tomography. 11.3.Time-domain
system. 11.4.Direct-current system. 11.5.Frequency-domain system.
11.6.Frequency-domain theory: basics. 11.7.Frequency-domain theory: linear
image reconstruction. 11.8.Frequency-domain theory: general image
reconstruction. 11.9.Appendix 11.A. ART and SIRT. 11.10.References.
11.11.Further readings. 11.12.Problems.
12. PHOTOACOUSTIC TOMOGRAPHY.
12.1.Introduction. 12.2.Motivation for photoacoustic tomography. 12.3.Initial
photoacoustic pressure. 12.4.General photoacoustic equation. 12.5.General
forward solution. 12.6.Delta-pulse excitation of a slab. 12.7.Delta-pulse
excitation of a sphere. 12.8.Finite-duration pulse excitation of a thin slab.
12.9.Finite-duration pulse excitation of a small sphere. 12.10.Dark-field
confocal photoacoustic microscopy. 12.11.Synthetic aperture image
reconstruction. 12.12.General image reconstruction. 12.13.Appendix 12.A.
Derivation of acoustic wave equation. 12.14.Appendix 12.B. Green's function
approach. 12.15.References. 12.16.Further readings. 12.17.Problems.
13.
ULTRASOUND-MODULATED OPTICAL TOMOGRAPHY. 13.1.Introduction. 13.2.Mechanisms
of ultrasonic modulation of coherent light. 13.3.Time-resolved
frequency-swept UOT. 13.4.Frequency-swept UOT with parallel-speckle
detection. 13.5.Ultrasonically modulated virtual optical source.
13.6.Reconstruction-based UOT. 13.7.UOT with Fabry-Perot interferometry.
Problems. Reading. Furhter Reading. APPENDIX A. DEFINITIONS OF OPTICAL
PROPERTIES. APPENDIX B. List of Acronyms. Index.
Lihong V. Wang , PhD, is Gene K. Beare Distinguished Professor in the Department of Biomedical Engineering and Director of the Optical Imaging Laboratory at Washington University in St. Louis. Dr. Wang is Chair of the International Biomedical Optics Society. His?Monte Carlo model of photon transport in biological tissues has been used worldwide. He has published more than 120 peer-reviewed journal articles and patents. HSIN-I WU , PhD, is Professor of Biomedical Engineering at Texas A&M University. He has published more than fifty peer-reviewed journal articles. Dr. Wu was a senior Fulbright scholar and is listed in Outstanding Educators of America. He serves on the Editorial Advisory Board of Biocomplexity and the Editorial Board of BioMedical Engineering OnLine.
