This book provides a detailed description of various multidimensional chromatographic separation techniques. The editor first provides an introduction to the area and then dives right into the various complex separation techniques. While still not used routinely comprehensive chromatography techniques will help acquaint the readers with the fundamentals and possible benefits of multi-dimensional separations coupled with mass spectrometry.
The topics include a wide range of material that will appease all interested in either entering the field of multidimensional chromatography and those looking to gain a better understanding of the topic.
This book provides a detailed description of various multidimensional chromatographic separation techniques. The editor first provides an introduction to the area and then dives right into the various complex separation techniques. While still not used routinely comprehensive chromatography techniques will help acquaint the readers with the fundamentals and possible benefits of multi-dimensional separations coupled with mass spectrometry.
The topics include a wide range of material that will appease all interested in either entering the field of multidimensional chromatography and those looking to gain a better understanding of the topic.
Arvustused
"It offers comprehensive coverage of the topic of comprehensive multidimensional separations, at a level that makes it a useful reference for scientists working in the field, or as a primary textbook for a graduate-level course on advanced separation techniques." ( Anal Bioanal Chem , 2012)
Contributors ix Preface xi 1 Introduction 1 Luigi Mondello, Peter
Q. Tranchida, and Keith D. Bartle 1.1 Two-Dimensional Chromatography-Mass
Spectrometry: A 50-Year-Old Combination, 2 1.2 Shortcomings of
One-Dimensional Chromatography, 3 1.3 Benefits of Two-Dimensional
Chromatography, 6 1.4 Book Content, 7 1.5 Final Considerations, 11 2
Multidimensional Gas Chromatography: Theoretical Considerations 13 Leonid
M. Blumberg 2.1 Symbols, 15 2.2 One-Dimensional GC, 17 2.3 Comprehensive GC
x GC, 31 3 Multidimensional Liquid Chromatography: Theoretical
Considerations 65 Pavel Jandera 3.1 Two-Dimensional LC Techniques, 66 3.2
Peak Capacity in HPLC: One- and Multidimensional Separations, 69 3.3
Orthogonality in Two-Dimensional LC-LC Systems, 73 3.4 Sample Dimensionality
and Structural Correlations, 75 3.5 Separation Selectivity and Selection of
Phase Systems in Two-Dimensional LC-LC, 79 3.6 Programmed Elution in
Two-Dimensional HPLC, 81 3.7 Fraction Transfer Modulation in Comprehensive LC
x LC: Additional Band Broadening, 87 3.8 Future Perspectives, 89 4 History,
Evolution, and Optimization Aspects of Comprehensive Two-Dimensional Gas
Chromatography 93 Ahmed Mostafa, Tadeusz G'orecki, Peter Q. Tranchida, and
Luigi Mondello 4.1 Fundamentals of GC x GC, 94 4.2 Modulation, 96 4.3 GC x
GC Data Interpretation, 97 4.4 GC x GC Instrumentation, 100 4.5 Thermal
Modulators, 101 4.6 Comprehensive Two-Dimensional GC Method Optimization, 115
4.7 Final Remarks, 140 5 Flow-Modulated Comprehensive Two-Dimensional Gas
Chromatography 145 John V. Seeley 5.1 Timing Requirements of GC x GC
Modulators, 145 5.2 Criteria for Evaluating Modulators, 150 5.3 Forms of
Modulation, 151 5.4 Single-Stage Flow Modulation, 152 5.5 Two-Stage Flow
Modulation, 157 5.6 Summary of Flow Modulators, 167 5.7 Brief Comparison to
Thermal Modulation, 169 5.8 Concluding Remarks, 169 6 Comprehensive
Two-Dimensional Gas Chromatography Combined with Mass Spectrometry 171
Peter Q. Tranchida, Luigi Mondello, Samuel D. H. Poynter, and Robert A.
Shellie 6.1 Instrument Requirements for GC x GC-MS, 173 6.2 Data Processing
of GC x GC-TOF MS Results, 180 6.3 Method Translation in GC x GC-MS, 181 6.4
GC x MS, 181 6.5 Conventional and Alternative Modulation Techniques for GC x
GC-MS, 184 6.6 GC x GC-MS Applications, 187 6.7 Concluding Remarks, 232 7
Detector Technologies and Applications in Comprehensive Two-Dimensional Gas
Chromatography 243 Philip J. Marriott 7.1 Detection in GC x GC, 244 7.2
Comments on GC x GC with Mass Spectrometry, 249 7.3 Flame Ionization
Detection in GC x GC, 250 7.4 Electron Capture Detection in GC x GC, 254 7.5
Sulfur Chemiluminescence Detection in GC x GC, 260 7.6 Nitrogen
Chemiluminescence Detection in GC x GC, 263 7.7 Atomic Emission Detection in
GC x GC, 265 7.8 Thermionic Detection in GC x GC, 267 7.9 Flame Photometric
Detection in GC x GC, 269 7.10 Case Study of GC x GC with Selective
Detection, 272 7.11 Dual Detection with GC x GC, 274 7.12 Conclusions, 275 8
History, Evolution, and Optimization Aspects of Comprehensive Two-Dimensional
Liquid Chromatography 281 Isabelle FranCcois, Koen Sandra, and Pat Sandra
8.1 Method Development and Instrumentation, 282 8.2 Technical Problems in
Comprehensive Liquid Chromatography, 309 8.3 Detection, 314 8.4 Data
Representation, 315 8.5 Instrumentation, 316 8.6 Milestones in Comprehensive
Liquid Chromatography, 317 8.7 Applications, 320 8.8 Beyond Two-Dimensional
Chromatography, 320 8.9 Comparison of LC x LC and Off-Line 2D LC, 321 8.10
Conclusions, 325 9 Comprehensive Two-Dimensional Liquid Chromatography
Combined with Mass Spectrometry 331 Paola Dugo, Luigi Mondello, Francesco
Cacciola, and Paola Donato 9.1 HPLC-MS, 332 9.2 LC x LC-MS Instrumentation
and Method Development, 334 9.3 LC x LC-MS Applications, 336 10
Comprehensive Two-Dimensional Liquid Chromatography Applications 391 Paola
Dugo, Luigi Mondello, Francesco Cacciola, and Paola Donato 10.1
Comprehensive 2D LC Separation of Synthetic and Natural Polymers, 392 10.2
Comprehensive 2D LC Separation of Natural Products and Antioxidants, 399 10.3
Comprehensive 2D LC Separation of Pharmaceutical and Environmental Compounds,
412 10.4 Comprehensive 2D LC Separation of Proteins and Peptides, 417 11
Other Comprehensive Chromatography Methods 429 Isabelle FranCcois, Pat
Sandra, Danilo Sciarrone, and Luigi Mondello 11.1 Online Two-Dimensional
Liquid Chromatography-Gas Chromatography, 430 11.2 Online Two-Dimensional
Supercritical Fluid Chromatography-Gas Chromatography, 440 11.3 Online
Two-Dimensional Supercritical Fluid Chromatography-Supercritical Fluid
Chromatography, 440 11.4 Online Two-Dimensional Supercritical Fluid
Chromatography-Liquid Chromatography, 442 12 Comprehensive Chromatography
Data Interpretation Technologies 449 Elizabeth M. Humston and Robert E.
Synovec 12.1 Higher-Order Data Structure, 450 12.2 Modifications of
First-Order Data-Handling Approaches, 453 12.3 Visualization, 456 12.4 Mass
Spectral Detection, 461 12.5 Chemometrics, 463 12.6 Summary of Data
Interpretation Technologies, 472 Index 477
Luigi Mondello is an expert in the field of multidimensional chromatography. He has published a number of books and papers in his field and has been an invited speaker at a number of conferences. He is Editor of the Journal of Separation Science (Wiley). Recently he was awarded The Chromatographic Society's Silver Jubilee Medal award for his work in the field of chromatography and the COLACRO Medal for his contribution to the development and diffusion of the Chromatographic Techniques, and the HTC-Award for the most outstanding and innovative work in the field of hyphenated chromatographic techniques from the Flemish Chemical Society. He is currently Full Professor of Analytical Chemistry at the the University of Messina in Italy and permanent member of the scientific committee of the International Symposium on Capillary Chromatography (ISCC); the International Symposium on Essential Oils (ISEO); the International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers (HTC-11); the Brazilian Symposium on Chromatography and Related Techniques (SIMCRO); and the Congresso Latino-Americano de Cromatografia e Tecnicas Relacionades (COLACRO).
