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Cryptosporidium: Methods and Protocols 2020 ed. [Pehme köide]

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  • Formaat: Paperback / softback, 407 pages, kõrgus x laius: 254x178 mm, kaal: 802 g, 71 Illustrations, color; 22 Illustrations, black and white; XI, 407 p. 93 illus., 71 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2052
  • Ilmumisaeg: 27-Aug-2020
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493997505
  • ISBN-13: 9781493997503
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  • Formaat: Paperback / softback, 407 pages, kõrgus x laius: 254x178 mm, kaal: 802 g, 71 Illustrations, color; 22 Illustrations, black and white; XI, 407 p. 93 illus., 71 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2052
  • Ilmumisaeg: 27-Aug-2020
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493997505
  • ISBN-13: 9781493997503
This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.

The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Cryptosporidium Diagnostic Assays: Microscopy.- Cryptosporidium
Diagnostic Assays: Molecular Detection.- Detection of Cryptosporidium
Recovered From Large-Volume Water Samples Using Dead-End
Ultrafiltration.- Cryptosporidium Oocyst Purification Using Discontinuous
Gradient Centrifugation.- Measuring Cryptosporidium Serologic Responses by
Multiplex Bead Assay.- Production and Purification of Functional
Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma
gondii.- Cryptosporidium Genotyping for Epidemiology Tracking.- Use of
Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and
Detection.- Preparation of Cryptosporidium DNA for Whole Genome Sequencing.-
Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.org.- A Novel
Method to Silence Genes in Cryptosporidium.- Use of miRNAs to Study Host
Cell-Parasite Interactions.- Genetic Manipulation of Cryptosporidium parvum
with CRISPR/Cas9.- Mouse Models for Use in Cryptosporidium Infection Studies
and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and
Histopathology.- Calf Clinical Model of Cryptosporidiosis for Efficacy
Evaluation of Therapeutics.- Cell Culture Infectivity to Assess Chlorine
Disinfection of Cryptosporidium Oocysts in Water.- High-Content Screening for
Cryptosporidium Drug Discovery.- High-Throughput Screening of Drugs Against
the Growth of Cryptosporidium parvum In Vitro by qRT-PCR.- In Vitro Culture
of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for
Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test
Compounds.- In Vitro Culture of Cryptosporidium parvum Using Stem
Cell-Derived Intestinal Epithelial Monolayers.- Two and Three Dimensional
Bioengineered Human Intestinal Tissue Models for Cryptosporidium.