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Preface | v | ||
Contributors | ix | ||
1 Cryptosporidium Diagnostic Assays: Microscopy | 1 | (10) | |
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2 Cryptosporidium Diagnostic Assays: Molecular Detection | 11 | (12) | |
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3 Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration | 23 | (20) | |
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4 Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation | 43 | (18) | |
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5 Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay | 61 | (26) | |
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6 Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii | 87 | (16) | |
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7 Cryptosporidium Genotyping for Epidemiology Tracking | 103 | (14) | |
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8 Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection | 117 | (12) | |
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9 Preparation of Cryptosporidium DNA for Whole Genome Sequencing | 129 | (10) | |
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10 Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org | 139 | (54) | |
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11 A Novel Method to Silence Genes in Cryptosporidium | 193 | (12) | |
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12 Use of miRNAs to Study Host Cell-Parasite Interactions | 205 | (14) | |
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13 Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9 | 219 | (10) | |
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14 Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology | 229 | (24) | |
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15 Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics | 253 | (30) | |
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16 Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water | 283 | (20) | |
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17 High-Content Screening for Cryptosporidium Drug Discovery | 303 | (16) | |
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18 High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR | 319 | (16) | |
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19 In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds | 335 | (16) | |
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20 In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers | 351 | (22) | |
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21 Two- and Three-Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium | 373 | (30) | |
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Index | 403 |