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Endocannabinoid Signaling: Methods and Protocols Second Edition 2023 [Pehme köide]

  • Formaat: Paperback / softback, 509 pages, kõrgus x laius: 254x178 mm, kaal: 994 g, 65 Illustrations, color; 73 Illustrations, black and white; XVII, 509 p. 138 illus., 65 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2576
  • Ilmumisaeg: 25-Sep-2023
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071627309
  • ISBN-13: 9781071627303
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  • Formaat: Paperback / softback, 509 pages, kõrgus x laius: 254x178 mm, kaal: 994 g, 65 Illustrations, color; 73 Illustrations, black and white; XVII, 509 p. 138 illus., 65 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2576
  • Ilmumisaeg: 25-Sep-2023
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071627309
  • ISBN-13: 9781071627303
This second edition provides new and updated chapters detailing all major elements of the ECB system. Chapters guide readers through identification of drug targets, electrophysiology, computational chemistry, and machine learning. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.





 Comprehensive and cutting-edge, Endocannabinoid Signaling: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.
Need for Methods to Investigate Endocannabinoid Signaling.- Extraction
and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-like
Lipids in Biological Tissues.- Measuring the Content of Endocannabinoid-like
Compounds in Biological Fluids: A Critical Overview of Sample Preparation
Methodologies.- LC-MS/MS Analysis of AEA and 2-AG.- Analysis of Omega-3 Fatty
Acid Derived N-Acylethanolamines in Biological
Matrices.- Endocannabinoid-Binding Receptors as Drug Targets.- Assay of CB1
Receptor Binding.- The Displacement Binding Assay Using Human Cannabinoid CB2
Receptor-Transfected Cells.- Fluorescence-based Assay for TRPV1
Channels.- DNA-Protein-Interaction (DPI)-ELISA Assay for PPAR- Receptor
Binding.- Scintillation Proximity Assay (SPA)-Based Radioligand Binding for
PPAR, PPAR and PPAR Receptors.- A Homogeneous Time-Resolved Fluorescence
Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPAR
andPPAR.- The Cyclic AMP Assay Using Human Cannabinoid CB2
Receptor-Transfected Cells.- Assay of GTPS Binding in
Autoradiography.- Cellular Assay to Study -Arrestin Recruitment by the
Cannabinoid Receptors 1 and 2.- Endocannabinoid Metabolism and Transport as
Drug Targets.- Assay of NAT Activity.- Radiometric Assay of NAPE-PLD
activity.- A Fluorescence-based NAPE-PLD Activity Assay.- Radiometric Assay
of FAAH Activity.- Fluorimetric Assay of FAAH activity.- Assay of NAAA
Activity.- Assay of DAGL/ Activity.- Assay of Monoacylglycerol Lipase
Activity.- Radiometric Assay of ABHD2 Activity.- Oxygenation of Anandamide by
Lipoxygenases.- Assay of Endocannabinoid Oxidation by Cytochrome
P450.- Assays of Endocannabinoid Uptake.- Assessing Gene Expression of the
Endocannabinoid System Components by Real-Time Quantitative Reverse
Transcription Polymerase Chain Reaction.- Bioinformatics of the
Endocannabinoid System: Study of DNA methylation at Rat Cnr1 gene
promoter.- DNA Methylation Analysis of Cnr1 Gene Promoter.- Western Blotting
of the Endocannabinoid System.- Quantitation of Plasma Membrane (G
Protein-Coupled) Receptor Trafficking in Cultured Cells.- Assessing CB1
Expression in the Brain by Immunohistochemical Methods: Light, Confocal and
Electron Microscopy.- Measuring Endocannabinoid System Interaction with
Biomembranes.- STORM Super-Resolution Imaging of CB1 Receptors in Tissue
Preparations.- Visualization of Endocannabinoids in the
Cell.- Electrophysiology of Endocannabinoid Signalling.- Machine Learning and
Computational Chemistry for the Endocannabinoid System.- A Virtual Reverse
Screening Approach to Target Type 2 Cannabinoid Receptor.