E-raamat: Endocannabinoid Signaling: Methods and Protocols

Need for Methods to Investigate Endocannabinoid Signaling.- Extraction
and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-like
Lipids in Biological Tissues.- Measuring the Content of Endocannabinoid-like
Compounds in Biological Fluids: A Critical Overview of Sample Preparation
Methodologies.- LC-MS/MS Analysis of AEA and 2-AG.- Analysis of Omega-3 Fatty
Acid Derived N-Acylethanolamines in Biological
Matrices.- Endocannabinoid-Binding Receptors as Drug Targets.- Assay of CB1
Receptor Binding.- The Displacement Binding Assay Using Human Cannabinoid CB2
Receptor-Transfected Cells.- Fluorescence-based Assay for TRPV1
Channels.- DNA-Protein-Interaction (DPI)-ELISA Assay for PPAR- Receptor
Binding.- Scintillation Proximity Assay (SPA)-Based Radioligand Binding for
PPAR, PPAR and PPAR Receptors.- A Homogeneous Time-Resolved Fluorescence
Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPAR
andPPAR.- The Cyclic AMP Assay Using Human Cannabinoid CB2
Receptor-Transfected Cells.- Assay of GTPS Binding in
Autoradiography.- Cellular Assay to Study -Arrestin Recruitment by the
Cannabinoid Receptors 1 and 2.- Endocannabinoid Metabolism and Transport as
Drug Targets.- Assay of NAT Activity.- Radiometric Assay of NAPE-PLD
activity.- A Fluorescence-based NAPE-PLD Activity Assay.- Radiometric Assay
of FAAH Activity.- Fluorimetric Assay of FAAH activity.- Assay of NAAA
Activity.- Assay of DAGL/ Activity.- Assay of Monoacylglycerol Lipase
Activity.- Radiometric Assay of ABHD2 Activity.- Oxygenation of Anandamide by
Lipoxygenases.- Assay of Endocannabinoid Oxidation by Cytochrome
P450.- Assays of Endocannabinoid Uptake.- Assessing Gene Expression of the
Endocannabinoid System Components by Real-Time Quantitative Reverse
Transcription Polymerase Chain Reaction.- Bioinformatics of the
Endocannabinoid System: Study of DNA methylation at Rat Cnr1 gene
promoter.- DNA Methylation Analysis of Cnr1 Gene Promoter.- Western Blotting
of the Endocannabinoid System.- Quantitation of Plasma Membrane (G
Protein-Coupled) Receptor Trafficking in Cultured Cells.- Assessing CB1
Expression in the Brain by Immunohistochemical Methods: Light, Confocal and
Electron Microscopy.- Measuring Endocannabinoid System Interaction with
Biomembranes.- STORM Super-Resolution Imaging of CB1 Receptors in Tissue
Preparations.- Visualization of Endocannabinoids in the
Cell.- Electrophysiology of Endocannabinoid Signalling.- Machine Learning and
Computational Chemistry for the Endocannabinoid System.- A Virtual Reverse
Screening Approach to Target Type 2 Cannabinoid Receptor.