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Mitochondrial Regulation: Methods and Protocols Second Edition 2021 [Pehme köide]

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  • Formaat: Paperback / softback, 318 pages, kõrgus x laius: 254x178 mm, kaal: 633 g, 47 Illustrations, color; 17 Illustrations, black and white; XII, 318 p. 64 illus., 47 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2310
  • Ilmumisaeg: 07-Jun-2021
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071614355
  • ISBN-13: 9781071614358
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  • Formaat: Paperback / softback, 318 pages, kõrgus x laius: 254x178 mm, kaal: 633 g, 47 Illustrations, color; 17 Illustrations, black and white; XII, 318 p. 64 illus., 47 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2310
  • Ilmumisaeg: 07-Jun-2021
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071614355
  • ISBN-13: 9781071614358
Teised raamatud teemal:
This fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics.
Isolation of Mitochondria from Liver and Extraction of Total RNA and
Protein: Analyses of miRNA and Protein Expression.- Determination of
Oxidative Phosphorylation Complexes Activities.- BN-PAGE-Based Approach to
Study Thyroid Hormones and Mitochondrial Function.- Monitoring Mitochondrial
Function in Mouse Embryonic Stem Cells (mESCs).- Mitochondrial Functional
Assessment in Mammalian Gametes Using Fluorescent Probes.- Update on the
Histoenzymatic Methods for Visualization of the Activity of Individual
Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections.- An
Update on Isolation of Functional Mitochondria from Cell for Bioenergetics
Studies.- PCR-Based Determination of Mitochondrial DNA Copy Number in
Multiple Species.- Methods to Monitor Mitophagy and Mitochondrial Quality:
Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases.-
Analysis of Pro-Apoptotic Protein Trafficking to and from Mitochondria.-
Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests:
Implications in Clinical Biochemistry.- Protocols for Mitochondria as the
Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver
Disease (NAFLD).- The Use of Reactive Oxygen Species Production by
Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in
Isolated Brown Adipose Tissue Mitochondria.- Mitochondrial Regulation
Assessment by 13C-NMR Isotopomer Analysis.- Assays for Determination of
Cellular and Mitochondrial NAD+ and NADH Content.- Following the Dynamism of
the Mitochondrial Network in T Cells.- Measuring PGC-1 and Its Acetylation
Status in Mouse Primary Myotubes.