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Pathogenic Yersinia: Methods and Protocols 2019 ed. [Pehme köide]

  • Formaat: Paperback / softback, 259 pages, kõrgus x laius: 254x178 mm, kaal: 522 g, 40 Illustrations, color; 6 Illustrations, black and white; XI, 259 p. 46 illus., 40 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2010
  • Ilmumisaeg: 14-Aug-2020
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 149399543X
  • ISBN-13: 9781493995431
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  • Formaat: Paperback / softback, 259 pages, kõrgus x laius: 254x178 mm, kaal: 522 g, 40 Illustrations, color; 6 Illustrations, black and white; XI, 259 p. 46 illus., 40 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2010
  • Ilmumisaeg: 14-Aug-2020
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 149399543X
  • ISBN-13: 9781493995431
Teised raamatud teemal:
This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols provides a single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.

Arvustused

The book is written for the research community, including novices. Clinicians and parasitologists with an interest in investigation and discovery of Yersinia virulence mechanisms may also find this book helpful and interesting. This is a wonderful resource for understanding the investigation and discovery of the virulence mechanisms of Yersinia. This book is quite unique in covering the methods and protocols related to Yersinia pathogenhost interactions. (Omer Iqbal, Doody's Book Reviews, November 01, 2019)

An intradermal model for Yersinia pestis inoculation.- Intranasal
Inoculation of Mice with Yersinia pestis and Processing of Pulmonary Tissue
for Analysis.- Standardized method for aerosol challenge of rodents with
Yersinia pestis for modeling primary pneumonic plague.- Mouse Models of
Yersiniosis.- Intravital Confocal Microscopy of Dermal Innate Immune
Responses to Flea-transmitted Yersinia pestis.- Laser scanning microscopy of
Yersinia pestis infected tissues.- Real-time monitoring of Yersinia pestis
promoter activity by bioluminescence imaging.- Discovering Yersinia-Host
Interactions by Tissue Dual RNA-seq.- Detection of cells translocated with
Yersinia Yops in infected tissues using -lactamase fusions.- Monitoring of
neutrophil recruitment to mice lungs during pneumonic plague.- Co-infection
assay to determine Yersinia pestis competitive fitness in fleas.- Drosophila
as a model for understanding the insect host of Yersinia pestis.-
Intracellular assays to monitor survival and growth of Yersinia pestis within
macrophages.- Human neutrophil isolation and degranulation responses to
Yersinia pestis infection.- Measurement of Yersinia translocon pore formation
in erythrocytes.- Analysis of inflammasome activation in response to Yersinia
infection by fluorescence microscopy detection of active caspase-1 puncta.-
Methods for detection of pyrin inflammasome assembly in macrophages infected
with Yersinia spp.