Muutke küpsiste eelistusi

E-raamat: Pathogenic Yersinia: Methods and Protocols

  • Formaat - PDF+DRM
  • Hind: 122,88 €*
  • * hind on lõplik, st. muud allahindlused enam ei rakendu
  • Lisa ostukorvi
  • Lisa soovinimekirja
  • See e-raamat on mõeldud ainult isiklikuks kasutamiseks. E-raamatuid ei saa tagastada.

DRM piirangud

  • Kopeerimine (copy/paste):

    ei ole lubatud

  • Printimine:

    ei ole lubatud

  • Kasutamine:

    Digitaalõiguste kaitse (DRM)
    Kirjastus on väljastanud selle e-raamatu krüpteeritud kujul, mis tähendab, et selle lugemiseks peate installeerima spetsiaalse tarkvara. Samuti peate looma endale  Adobe ID Rohkem infot siin. E-raamatut saab lugeda 1 kasutaja ning alla laadida kuni 6'de seadmesse (kõik autoriseeritud sama Adobe ID-ga).

    Vajalik tarkvara
    Mobiilsetes seadmetes (telefon või tahvelarvuti) lugemiseks peate installeerima selle tasuta rakenduse: PocketBook Reader (iOS / Android)

    PC või Mac seadmes lugemiseks peate installima Adobe Digital Editionsi (Seeon tasuta rakendus spetsiaalselt e-raamatute lugemiseks. Seda ei tohi segamini ajada Adober Reader'iga, mis tõenäoliselt on juba teie arvutisse installeeritud )

    Seda e-raamatut ei saa lugeda Amazon Kindle's. 

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols provides a single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.

Arvustused

The book is written for the research community, including novices. Clinicians and parasitologists with an interest in investigation and discovery of Yersinia virulence mechanisms may also find this book helpful and interesting. This is a wonderful resource for understanding the investigation and discovery of the virulence mechanisms of Yersinia. This book is quite unique in covering the methods and protocols related to Yersinia pathogenhost interactions. (Omer Iqbal, Doody's Book Reviews, November 01, 2019)

Preface v
Contributors ix
Part I Mouse Models For Yersinia Infection
1 An Intradermal Model for Yersinia pestis Inoculation
3(14)
Rodrigo J. Gonzalez
2 Intranasal Inoculation of Mice with Yersinia pestis and Processing of Pulmonary Tissue for Analysis
17(12)
Roger D. Pechous
3 Standardized Method for Aerosol Challenge of Rodents with Yersinia pestis for Modeling Primary Pneumonic Plague
29(12)
Paul E. Anderson
Rachel M. Olson
Joshua L. Willix
Deborah M. Anderson
4 Mouse Models of Yersiniosis
41(16)
Diana Hooker-Romero
Leah Schwiesow
Yahan Wei
Victoria Auerbuch
Part II Monitoring Bacteria During Infection
5 Intravital Confocal Microscopy of Dermal Innate Immune Responses to Flea-Transmitted Yersinia pestis
57(12)
Jeffrey G. Shannon
B. Joseph Hinnebusch
6 Laser Scanning Microscopy of Yersinia pestis Infected Tissues
69(16)
Rodrigo J. Gonzalez
7 Real-Time Monitoring of Yersinia pestis Promoter Activity by Bioluminescence Imaging
85(14)
Anne Derbise
Olivier Dussurget
Elisabeth Carniel
Javier Pizarro-Cerda
8 Discovering Yersinia-Host Interactions by Tissue Dual RNA-Seq
99(18)
Maria Kusmierek
Ann Kathrin Heroven
Michael Beckstette
Aaron M. Nuss
Petra Dersch
9 Detection of Cells Translocated with Yersinia Yops in Infected Tissues Using β-Lactamase Fusions
117(24)
Giang T. Nguyen
Anne L. McCabe
Alyssa C. Fasciano
Joan Mecsas
10 Monitoring of Neutrophil Recruitment to Mice Lungs During Pneumonic Plague
141(12)
Yaron Vagima
Yinon Levy
Emanuelle Mamroud
Part III Invertebrate Models Of Infection (In Vivo And In Vitro)
11 Co-infection Assay to Determine Yersinia pestis Competitive Fitness in Fleas
153(14)
Athena Lemon
Amelia Silva-Rohwer
Janelle Sagawa
Viveka Vadyvaloo
12 Drosophila as a Model for Understanding the Insect Host of Yersinia pestis
167(14)
Anna B. Ludlow
Cassandra D. Pauling
Melanie M. Marketon
Deborah M. Anderson
Part IV Yersinia Interaction With Immune Cells And Immune Signaling
13 Intracellular Assays to Monitor Survival and Growth of Yersinia pestis Within Macrophages
181(16)
Amanda R. Pulsifer
Tiva T. VanCleave
Matthew B. Lawrenz
14 Human Neutrophil Isolation and Degranulation Responses to Yersinia pestis Infection
197(14)
Kara R. Eichelberger
William E. Goldman
15 Measurement of Yersinia Translocon Pore Formation in Erythrocytes
211(20)
Tiago R.D. Costa
Monika K. Francis
Salah I. Farag
Tomas Edgren
Matthew S. Francis
16 Analysis of Inflammasome Activation in Response to Yersinia Infection by Fluorescence Microscopy Detection of Active Caspase-1 Puncta
231(10)
Erin E. Zwack
Igor E. Brodsky
17 Methods for Detection of Pyrin Inflammasome Assembly in Macrophages Infected with Yersinia spp
241(16)
Natasha P. Medici
James B. Bliska
Index 257