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Phase-Separated Biomolecular Condensates: Methods and Protocols 2023 ed. [Kõva köide]

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  • Formaat: Hardback, 449 pages, kõrgus x laius: 254x178 mm, kaal: 1063 g, 1 Illustrations, black and white; XIV, 449 p. 1 illus., 1 Hardback
  • Sari: Methods in Molecular Biology 2563
  • Ilmumisaeg: 14-Oct-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071626620
  • ISBN-13: 9781071626627
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Phase-Separated Biomolecular Condensates: Methods and Protocols 2023 ed.Suurem pilt
  • Formaat: Hardback, 449 pages, kõrgus x laius: 254x178 mm, kaal: 1063 g, 1 Illustrations, black and white; XIV, 449 p. 1 illus., 1 Hardback
  • Sari: Methods in Molecular Biology 2563
  • Ilmumisaeg: 14-Oct-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071626620
  • ISBN-13: 9781071626627
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9781071626627.html
Märksõnad:

This volume provides readers with a broad collection of theoretical, computational, and experimental methods to quantitatively study the properties of phase-separate biomolecular condensates in diverse systems. The chapters in this book cover topics such as theoretical and computational methods; methods for in vitro characterization of biomolecular condensates; and techniques that enable in-cell characterization of biomolecular condensates. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Phase-Separated Biomolecular Condensates: Methods and Protocols is a valuable resource that helps researchers learn and use established methods to study both biophysical properties and biological functions of biomolecular condensates. 



Calculating Binodals and Interfacial Tension of Phase-Separated
Condensates from Molecular Simulations, with Finite-Size
Corrections.- Field-Theoretic Simulation Method to Study the Liquid-Liquid
Phase Separation of Polymers.- Numerical Techniques for Applications of
Analytical Theories to Sequence-Dependent Phase Separations of Intrinsically
Disordered Proteins.- An Introduction to the Stickers-and-Spacers Framework
as Applied to Biomolecular Condensates.- Multi-Scale Modeling of Protein-RNA
Condensation In and Out of Equilibrium.- Fluorescence Lifetime Imaging
Microscopy of Biomolecular Condensates.- Single-Molecule Fluorescence Methods
to Study Protein-RNA Interactions Underlying Biomolecular
Condensates.- Fluorescence Correlation Spectroscopy and Phase
Separation.- Measurement of Protein and Nucleic Acid Diffusion Coefficients
within Biomolecular Condensates Using In-Droplet Fluorescence Correlation
Spectroscopy.- Single-Molecule Imaging of the Phase Separation-Modulated DNA
Compaction to Study Transcriptional Repression.- Phase Separation-Based
Biochemical Assays for Biomolecular Interactions.- Determining Thermodynamic
and Material Properties of Biomolecular Condensates by Confocal Microscopy
and Optical Tweezers.- A High-Throughput Method to Profile Protein
Liquid-Liquid Phase Separation.- Phase Separation of Rubisco by the Folded
SSUL Domains of CcmM in Beta-Carboxysome Biogenesis.- Cryo-Electron
Tomography of Reconstituted Biomolecular Condensate.- Sedimentation Assays to
Assess the Impact of Post-Translational Modifications on Phase Separation of
RNA-Binding Proteins In Vitro and In Cells.- Synthetic Organelles for
Multiple mRNA Selective Genetic Code Expansion in Eukaryotes.- Single
Molecule Tracking of RNA Polymerase In and Out of Condensates in Live
Bacterial Cells.- An Optogenetic Toolkit for the Control of Phase Separation
in Living Cells.- Assessing the Phase Separation Propensity of Proteins in
Living Cells Through Optodroplet Formation.- Mass Balance Imaging: A Phase
Portrait Analysis for Characterizing Growth Kinetics of Biomolecular
Condensates.- Characterizing Properties of Biomolecular Condensates Below the
Diffraction Limit In Vivo.