E-raamat: Phase-Separated Biomolecular Condensates: Methods and Protocols

Calculating Binodals and Interfacial Tension of Phase-Separated
Condensates from Molecular Simulations, with Finite-Size
Corrections.- Field-Theoretic Simulation Method to Study the Liquid-Liquid
Phase Separation of Polymers.- Numerical Techniques for Applications of
Analytical Theories to Sequence-Dependent Phase Separations of Intrinsically
Disordered Proteins.- An Introduction to the Stickers-and-Spacers Framework
as Applied to Biomolecular Condensates.- Multi-Scale Modeling of Protein-RNA
Condensation In and Out of Equilibrium.- Fluorescence Lifetime Imaging
Microscopy of Biomolecular Condensates.- Single-Molecule Fluorescence Methods
to Study Protein-RNA Interactions Underlying Biomolecular
Condensates.- Fluorescence Correlation Spectroscopy and Phase
Separation.- Measurement of Protein and Nucleic Acid Diffusion Coefficients
within Biomolecular Condensates Using In-Droplet Fluorescence Correlation
Spectroscopy.- Single-Molecule Imaging of the Phase Separation-Modulated DNA
Compaction to Study Transcriptional Repression.- Phase Separation-Based
Biochemical Assays for Biomolecular Interactions.- Determining Thermodynamic
and Material Properties of Biomolecular Condensates by Confocal Microscopy
and Optical Tweezers.- A High-Throughput Method to Profile Protein
Liquid-Liquid Phase Separation.- Phase Separation of Rubisco by the Folded
SSUL Domains of CcmM in Beta-Carboxysome Biogenesis.- Cryo-Electron
Tomography of Reconstituted Biomolecular Condensate.- Sedimentation Assays to
Assess the Impact of Post-Translational Modifications on Phase Separation of
RNA-Binding Proteins In Vitro and In Cells.- Synthetic Organelles for
Multiple mRNA Selective Genetic Code Expansion in Eukaryotes.- Single
Molecule Tracking of RNA Polymerase In and Out of Condensates in Live
Bacterial Cells.- An Optogenetic Toolkit for the Control of Phase Separation
in Living Cells.- Assessing the Phase Separation Propensity of Proteins in
Living Cells Through Optodroplet Formation.- Mass Balance Imaging: A Phase
Portrait Analysis for Characterizing Growth Kinetics of Biomolecular
Condensates.- Characterizing Properties of Biomolecular Condensates Below the
Diffraction Limit In Vivo.