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Practical Handbook on Soil Protists 2024 ed. [Kõva köide]

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  • Formaat: Hardback, 205 pages, kõrgus x laius: 254x178 mm, 13 Illustrations, color; 6 Illustrations, black and white; XII, 205 p. 19 illus., 13 illus. in color., 1 Hardback
  • Sari: Springer Protocols Handbooks
  • Ilmumisaeg: 20-Mar-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071637495
  • ISBN-13: 9781071637494
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Practical Handbook on Soil Protists 2024 ed.Suurem pilt
  • Formaat: Hardback, 205 pages, kõrgus x laius: 254x178 mm, 13 Illustrations, color; 6 Illustrations, black and white; XII, 205 p. 19 illus., 13 illus. in color., 1 Hardback
  • Sari: Springer Protocols Handbooks
  • Ilmumisaeg: 20-Mar-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071637495
  • ISBN-13: 9781071637494
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9781071637494.html
Märksõnad:
This volume details a wide range of basic and advanced techniques associated with research on isolation of important soil protozoa, and identification. Chapters guide readers through calculations of soil protists, basic techniques for isolation, enumeration, enrichment, molecular techniques, and staining techniques.





 





Authoritative and cutting-edge, Practical Handbook on Soil Protists aims to ensure successful results in the further study of this vital field.
Soil sampling, processing and storage.- Initial observation of protist
from soil.- Abundance calculation of naked and testate amoebae from
soil.- Abundance calculation of mycophagous amoebae.- Abundance calculation
of ciliates from soilAbundance calculation of flagellates from
soil.- Methodology to study species diversity of naked amoeba and testate
amoeba.- Non-flooded Petri plate method to study the species diversity of
flagellates.- Enumeration of testate amoeba through direct count from
soil.- Most Probable Number (MPN) method for enumeration of soil
protists.- Standard solutions and media used for isolation of soil
protists.- Isolation techniques for amoeba.- Isolation of ciliates and
flagellates from soil.- Isolation and Enumeration of Mycophagous
Protist.- Media used for enrichment of soil protists.- Enrichment of naked
amoebae species.- Enrichment of ciliates and flagellates.- Enrichment of
mycophagous protists.- Mono-axenic cultivation of protists.- Axenic
cultivation of soil protists.- Classical wet mount method for observing live
protists.- SEM: Sample Preparation, Fixation and Staining of Protists.- TEM:
Sample Preparation, Fixation and Staining of Protists.- Protargol staining
technique for identification of soil protists.-FLUTAX Staining of
cilliates.- DNA extraction from trophozoites and cysts using manual
protocol.- DNA isolation from single ciliate isolate.- Species identification
through sequencing.- DNA Barcoding techniques for Protist.- Unequivocal
identification through fluorescence in situ hybridization (FISH)
technique.- Isolation, DNA extraction, amplification, and gel electrophoresis
of single-celled non-marine foraminifera (Rhizaria).- Isolation and
cultivation and staining of Paramecium.- Isolation and cultivation of Euglena.