E-raamat: Practical Handbook on Soil Protists

Soil sampling, processing and storage.- Initial observation of protist
from soil.- Abundance calculation of naked and testate amoebae from
soil.- Abundance calculation of mycophagous amoebae.- Abundance calculation
of ciliates from soilAbundance calculation of flagellates from
soil.- Methodology to study species diversity of naked amoeba and testate
amoeba.- Non-flooded Petri plate method to study the species diversity of
flagellates.- Enumeration of testate amoeba through direct count from
soil.- Most Probable Number (MPN) method for enumeration of soil
protists.- Standard solutions and media used for isolation of soil
protists.- Isolation techniques for amoeba.- Isolation of ciliates and
flagellates from soil.- Isolation and Enumeration of Mycophagous
Protist.- Media used for enrichment of soil protists.- Enrichment of naked
amoebae species.- Enrichment of ciliates and flagellates.- Enrichment of
mycophagous protists.- Mono-axenic cultivation of protists.- Axenic
cultivation of soil protists.- Classical wet mount method for observing live
protists.- SEM: Sample Preparation, Fixation and Staining of Protists.- TEM:
Sample Preparation, Fixation and Staining of Protists.- Protargol staining
technique for identification of soil protists.-FLUTAX Staining of
cilliates.- DNA extraction from trophozoites and cysts using manual
protocol.- DNA isolation from single ciliate isolate.- Species identification
through sequencing.- DNA Barcoding techniques for Protist.- Unequivocal
identification through fluorescence in situ hybridization (FISH)
technique.- Isolation, DNA extraction, amplification, and gel electrophoresis
of single-celled non-marine foraminifera (Rhizaria).- Isolation and
cultivation and staining of Paramecium.- Isolation and cultivation of Euglena.