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Protein NMR Techniques 3rd ed. 2012 [Kõva köide]

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  • Formaat: Hardback, 518 pages, kõrgus x laius: 254x178 mm, kaal: 1186 g, XIV, 518 p., 1 Hardback
  • Sari: Methods in Molecular Biology 831
  • Ilmumisaeg: 14-Dec-2011
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1617794791
  • ISBN-13: 9781617794797
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  • Formaat: Hardback, 518 pages, kõrgus x laius: 254x178 mm, kaal: 1186 g, XIV, 518 p., 1 Hardback
  • Sari: Methods in Molecular Biology 831
  • Ilmumisaeg: 14-Dec-2011
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1617794791
  • ISBN-13: 9781617794797
In its expanded third edition, this Methods in Molecular Biology™ volume offers techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing, materials lists, step-by-step protocols, troubleshooting tips and more.

The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein NMR Techniques,Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR.
Preface v
Contributors xi
1 A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-Labeled Proteins
1(18)
Victoria Murray
Yuefei Huang
Jianglei Chen
Jianjun Wang
Qianqian Li
2 Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis
19(18)
Toshihiko Sugiki
Osamu Ichikawa
Mayumi Miyazawa-Onami
Ichio Shimada
Hideo Takahashi
3 Isotope Labeling in Insect Cells
37(18)
Krishna Saxena
Arpana Dutta
Judith Klein-Seetharaman
Harald Schwalbe
4 Isotope Labeling in Mammalian Cells
55(16)
Arpana Dutta
Krishna Saxena
Harald Schwalbe
Judith Klein-Seetharaman
5 Cell-Free Protein Production for NMR Studies
71(14)
Mitsuhiro Takeda
Masatsune Kainosho
6 Cell-Free Membrane Protein Expression for Solid-State NMR
85(26)
Alaa Abdine
Kyu-Ho Park
Dror E. Warschawski
7 Expression and Purification of Src-family Kinases for Solution NMR Studies
111(22)
Andrea Piserchio
David Cowburn
Ranajeet Ghose
8 NMR Studies of Large Protein Systems
133(8)
Shiou-Ru Tzeng
Ming-Tao Pai
Charalampos G. Kalodimos
9 Protein Dynamics by 15N Nuclear Magnetic Relaxation
141(24)
Fabien Ferrage
10 Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel
165(16)
Jolyon K. Claridge
Jason R. Schnell
11 Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy
181(16)
Chris A. Brosey
Marie-Eve Chagot
Walter J. Chazin
12 NMR Studies of Protein-RNA Interactions
197(22)
Carla A. Theimer
Nakesha L. Smith
May Khanna
13 Preparation and Optimization of Protein-DNA Complexes Suitable for Detailed NMR Studies
219(14)
My D. Sam
Robert T. Clubb
14 NMR Studies of Protein-Ligand Interactions
233(28)
Michael Goldflam
Teresa Tarrago
Margarida Gairi
Ernest Giralt
15 In-Cell NMR Spectroscopy in Escherichia coli
261(18)
Kirsten E. Robinson
Patrick N. Reardon
Leonard D. Spicer
16 Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS Solid-State NMR
279(24)
Bernd Reif
17 Solid-State NMR Spectroscopy of Protein Complexes
303(30)
Shangjin Sun
Yun Han
Sivakumar Paramasivam
Si Yan
Amanda E. Siglin
John C. Williams
In-Ja L. Byeon
Jinwoo Ahn
Angela M. Gronenborn
Tatyana Polenova
18 Synthesis, Purification, and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy
333(26)
Miki Itaya
Ian C. Brett
Steven O. Smith
19 Assignment of Backbone Resonances in a Eukaryotic Protein Kinase - ERK2 as a Representative Example
359(10)
Andrea Piserchio
Kevin N. Dalby
Ranajeet Ghose
20 Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility
369(38)
Griselda Hernandez
Janet S. Anderson
David M. LeMaster
21 Fast Protein Backbone NMR Resonance Assignment Using the BATCH Strategy
407(22)
Bernhard Brutscher
Ewen Lescop
22 Comprehensive Automation for NMR Structure Determination of Proteins
429(24)
Paul Guerry
Torsten Herrmann
23 ARIA for Solution and Solid-State NMR
453(32)
Benjamin Bardiaux
Therese Malliavin
Michael Nilges
24 Determining Protein Dynamics from 15N Relaxation Data by Using DYNAMICS
485(28)
David Fushman
Index 513