Chapter 1 Introduction
Chapter 2 Purification processes: analytical methods and enzyme stability
Chapter 3 Cell Disruption
Chapter 4 Filtration and Centrifugation
Chapter 5 Membrane Separation Processes
Chapter 6 Protein Precipitation
Chapter 7 Liquid-liquid extraction in aqueous two-phase systems
Chapter 8 Introduction to chromatography
Chapter 9 Size exclusion chromatography
Chapter 10 Iion Exchange chromatography
Chapter 11 Hydrophobic interaction chromatography
Chapter 12 Affinity Chromatography
Chapter 13 Adsorption chromatography in monolithic columns
Chapter 14 Chromatography: expanded bed adsorption (EBA)
Chapter 15 Continuous Chromatography using a Simulated Moving Bed System
Chapter 16 Chromatography scale-up
Chapter 17 Crystallization
Chapter 18 Distillation
Chapter 19 Plasmid Purification
Chapter 20 Purification of monoclonal antibodies
Chapter 21 Peptides of biotechnological interest: general concepts, production, purification and chemical characterization
Chapter 22 Integration of steps in the production of biotechnological products
Dr. Adalberto Pessoa Jr., Full professor at the School of Pharmaceutical Sciences, Biochemical-Pharmaceutical Technology Department, of the University of São Paulo and Visiting Professor at Kings College London, Institute of Pharmaceutical Science. He has experience in fermentation technology and in the purification processes of biotechnological products of interest to the pharmaceutical and food industries.
Dr. Beatriz Vahan Kilikian, nowadays retired, she was Associate Professor at the Polytechnic School of the University of São Paulo, from 1983 to 2012. She has experience in microrganisms cultivation and in the purification processes of biotechnological products of interest to the pharmaceutical, chemical and food industries.
Dr. Paul Frederick Long Full Professor of Biotechnology at King's College London and Visiting International Research Professor at the University of São Paulo. Prof Long is a microbiologist by training and his research uses a combination of bioinformatics, laboratory and field studies to discover new medicines from nature, particularly from the marine environment.
