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E-book: Imaging from Cells to Animals In Vivo

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"Imaging from Cells to Animals In Vivo offers an overview of optical imaging techniques developed over the last two decades to investigate biological processes in live cells and tissues. It comprehensively covers the main imaging approaches used, as wellas the application of those techniques to biological investigations in preclinical models. Among the areas covered are cell metabolism, receptor-ligand interactions, membrane trafficking, cell signaling, cell migration, cell adhesion, cytoskeleton and other processes using various molecular optical imaging techniques in living organisms, such as mice and zebrafish. Features Brings together biology and advanced optical imaging techniques to provide an overview of progress and modern methods from microscopy to whole body imaging Fills the need for a comprehensive view of application-driven development and use of new tools to ask new biological questions in the context of a living system. Includes basic chapters on key methods and instrumentation, from fluorescence microscopy and imaging to endoscopy, optical coherence tomography, and super-resolution imaging. Discusses approaches at different length scales and biomedical applications to the study of single cell, whole organ, and whole organism behavior. Addresses the impact on discovery, such as cellular function as implicated in human disease, and translational medicine, for example in cancer diagnosis"--

Imaging from Cells to Animals In Vivo offers an overview of optical imaging techniques developed over the last two decades to investigate biological processes in live cells and tissues. It comprehensively covers the main imaging approaches used, as well as the application of those techniques to biological investigations in preclinical models. Among the areas covered are cell metabolism, receptor-ligand interactions, membrane trafficking, cell signaling, cell migration, cell adhesion, cytoskeleton and other processes using various molecular optical imaging techniques in living organisms, such as mice and zebrafish.

Features

  • Brings together biology and advanced optical imaging techniques to provide an overview of progress and modern methods from microscopy to whole body imaging
  • Fills the need for a comprehensive view of application-driven development and use of new tools to ask new biological questions in the context of a living system.
  • Includes basic chapters on key methods and instrumentation, from fluorescence microscopy and imaging to endoscopy, optical coherence tomography, and super-resolution imaging
  • Discusses approaches at different length scales and biomedical applications to the study of single cell, whole organ, and whole organism behavior.
  • Addresses the impact on discovery, such as cellular function as implicated in human disease, and translational medicine, for example in cancer diagnosis.
    • Addresses the impact on discovery, such as cellular function as implicated in human disease, and translational medicine, for example in cancer diagnosis.
  • Preface vii
    The Editors ix
    List of Contributors
    xi
    Section I Overview of Imaging Methods and Instrumentation
    1 Fluorescence Microscopy Techniques
    3(14)
    Mehmet S. Ozturk
    Robert Prevedel
    2 Intravital Microscopy
    17(18)
    Mario Perm
    Jacky G. Goetz
    Antonio Peixoto
    3 An Introduction to Live-Cell Super-Resolution Imaging
    35(24)
    Sidn Culley
    Pedro Matos Pereira
    Romain F. Laine
    Ricardo Henriques
    4 Endoscopic Optical Coherence Tomography: Technologies and Applications
    59(16)
    Dawei Li
    Hyeon-Cheol Park
    Wu Yuan
    Xinwen Yao
    Xingde Li
    5 Bioluminescence
    75(16)
    Michael Conway
    Tingting Xu
    Amelia Brumbaugh
    Anna Young
    Dan Close
    Steven Ripp
    6 Macroscopic Fluorescence Imaging
    91(16)
    Anand T. N. Kumar
    7 Optical Coherence Tomography
    107(22)
    Roshan Dsouza
    Stephen A. Boppart
    8 Multiscale Photoacoustic Imaging
    129(12)
    Tianxiong Wang
    Song Hu
    9 Fluorescence Lifetime: Techniques, Analysis, and Applications in the Life Sciences
    141(30)
    Jenu Varghese Chacko
    Md Abdul Kader Sagar
    Kevin W. Eliceiri
    Section II Imaging Cellular Behavior
    10 Imaging Cell Metabolism: Analyzing Cellular Metabolism by NAD(P)H and FAD
    171(12)
    Ruofan Cao
    Horst Wallrabe
    Karsten H. Siller
    Ammasi Periasamy
    11 Intravital Imaging of Cancer Cell Migration In Vivo
    183(16)
    David Entenberg
    Maja H. Oktay
    John Condeelis
    12 Imaging Cellular Signaling In Vivo Using Fluorescent Protein Biosensors
    199(12)
    Christopher A. Reissaus
    Richard N. Day
    Kenneth W. Dunn
    13 Imaging Cell Adhesion and Migration
    211(10)
    Chandrani Mondal
    Julie Di Martino
    Jose Javier Bravo-Cordero
    14 Imaging the Living Eye
    221(22)
    Brian T. Soetikno
    Lisa Beckmann
    Hao F. Zhang
    Section III Whole-Organ and Whole-Organism Imaging
    15 Heart Imaging
    243(16)
    Leonardo Sacconi
    Claudia Crocini
    16 Visualizing Hepatic Immunity through the Eyes of Intravital Microscopy
    259(8)
    Maria Alice Freitas-Lopes
    Maisa Mota Antunes
    Raquel Carvalho-Gontijo
    Erika de Carvalho
    Gustavo Batista Menezes
    17 Optical Imaging of the Mammalian Oviduct In Vivo
    267(12)
    Shang Wang
    Irina V. Larina
    18 Immune System Imaging
    279(16)
    Michael J. Hickey
    M. Ursula Norman
    19 Imaging Living Organisms
    295(12)
    Masahiro Fukuda
    Katsuya Ozawa
    Hajime Hirase
    20 Live Imaging of Zebrafish
    307(18)
    Yinan Wan
    Philipp J. Keller
    Burkhard Hockendorf
    21 Whole-Body Fluorescence Imaging in Cancer Research
    325(12)
    Marina V. Shirmanova
    Diana V. Yuzhakova
    Maria M. Lukina
    Alena I. Gavrina
    Aleksandra V. Meleshina
    Hya V. Turchin
    Elena V. Zagaynova
    22 Large-Scale Fluorescence Imaging in Neuroscience
    337(12)
    Robert Prevedel
    Index 349
    Margarida Barroso is a Professor at the Department of Molecular and Cellular Physiology, Albany Medical College (Albany, NY).

    Xavier Intes is a Professor in the Biomedical Engineering Department and Co-Director of the Center for Modeling, Simulation and Imaging for Medicine (CeMSIM),at Rensselaer Polytechnic Institute (Troy, U.S.).