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E-raamat: Activity-Based Proteomics: Methods and Protocols

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This volume comprehensively covers techniques that use activity-based proteomics to investigate biological functions of proteins such as lipolytic enzymes, serine hydrolases, chitinases, and glycoside hydrolases. The chapters in this book cover a wide range of protocols and an overview on the application of activity-based proteomics profiling to study deISGylating enzymes, serine hydrolases, monooxygenases, ubiquitin E3 ligases, and reactive cysteine in various organisms, including bacteria, viruses, and mammalian cells and tissues. Furthermore, this book provides updated, comprehensive protocols for utilizing activity-based proteomics profiling to discover new enzymes, detect novel modifications, and identify new drug therapy targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble3.shooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Activity-Based Proteomics: Methods and Protocols is a valuable resource for all researchers who are interested in applying the latest technologies in this advancing field.

Activity-Based Protein Profiling (ABPP) of Cellular DeISGylating Enzymes
and Inhibitor Screening.-  Integrated Multi-Omics Approaches for Studying
Rare Genetic Diseases.- Activity-Based Protein Profiling of Serine Hydrolases
in Bacteria: Methods and Protocols.- Profiling Ligand-Induced Changes in
Nuclear Localization Using Proximity Labeling-Coupled Chemoproteomic.- An
Overview of Analytical Approaches to Cancer Proteogenomics.- Microplate-Based
Enzymatic Activity Assay Protocol Powered by Activity-Based Probes.-
Identifying Inhibitor Targets in Mycobacteria by Activity-Based Probe
Profiling.- Application of Activity-Based Protein Profiling (ABPP) Strategy
in the Identification of New Targets for Vascular Normalization and Binding
Site Resolution.- Activity-Based Protein Profiling to Study Virus-Host and
Viral-Induced MicroRNA-Host Interactions.- Activity-Based Protein Profiling
of Active Host Cell Serine Hydrolases for Bioprocess Development of
Therapeutic Proteins and Vaccines.- Synthesis and Application of
Activity-Based Probes for Serine Proteases.-  A Chemical Proteomics Workflow
to Profile Cellular Target Engagement of Kinase Inhibitors.- Isolation of
Exosome from Senescent Preadipocytes for Mass Spectrometric Analysis.-
Identification of Anticancer ROS Targets by Cysteine Reactivity Protein
Profiling.- A Comprehensive Data Processing Pipeline for Phosphoproteomics in
Viral Infection Studies.- Activity-Based Protein Profiling of Bacterial
Monooxygenases.- Activity-Based Profiling of Reactive Nucleophilic Cysteine
Sites from Tissue Proteomes for Drug Discovery Applications.- Activity-Based
Protein Profiling for Functional Cysteines and Protein Target
Identification.- System-Wide Profiling of Ubiquitin E3 Ligase Activities with
Quantitative Proteomics and Bioinformatics Analysis.- Recent Overview of
Protein Palmitoylation and Profiling Methodologies.