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E-raamat: ADAMTS Proteases: Methods and Protocols

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ADAMTS Proteases: Methods and ProtocolsSuurem pilt
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This volume provides methods for the analysis of ADAMTS proteases and ADAMTS-like proteins. Chapters detail ADAMTS family members, with special attention to the exciting progress made in understanding ADAMTS13its biology, disease mechanisms, detection, and clinical management of a potentially fatal thrombotic microangiopathy, thrombotic thrombocytopenic purpura (TTP). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, ADAMTS Proteases: Methods and Protocol is a valuable resource for any researcher hoping to contribute to the ever expanding world of ADAMTS.
An overview of ADAMTS proteases.- Overview of the Roles of ADAMTS1 in
Ovarian Biology.- Methods for Defining the Role of ADAMTS1 in the Ovary.-
Modeling Aspects of Human Placental Development with Trophoblast Stem Cells.-
Exploring The Role of ADAMTS4 Expression in Atherosclerosis.- Method for
Generation of adamtsl4 knock-out zebrafish lines using CRRISPR/Cas9 system.-
Assay for neurocan-degrading activity of  ADAMTS12  metalloprotease.-
Generating adamts13-/- Zebrafish via CRISPR/Cas9 Gene Editing.- Determining
ADAMTS13 conformation for the diagnosis of immune thrombotic thrombocytopenic
purpura and prediction of relapse.- Methods for studying the role of ADAMTS13
in angiogenesis.- Assays for Plasma ADAMTS13 Activity and Inhibitors using
recombinant FRETS-VWF73 peptide.- A Vortex-Shear Based Assay for Cleavage of
Multimeric VWF by ADAMTS13.- Deep protease profiling to define the substrate
specificity of ADAMTS proteases.- Microfluidic shear-based assay for
assessing ADAMTS13 activity under flow- OpticalTweezers SingleMolecule
Assay for ADAMTS13Mediated Proteolysis of the vonWillebrand Factor A2
Domain.- Method for Assessing Inhibition Kinetics of Autoantibodies Against
ADAMTS13 in Immune Thrombotic Thrombocytopenic Purpura.- Hydrogen Exchange
Mass Spectrometry for Mapping Antibody Binding Sites and Conformational
Dynamics of ADAMTS13.- Treating Immune Thrombotic Thrombocytopenic Purpura
with Therapeutic Plasma Exchange.- Molecular Dynamics Simulations with NAMD
and Trajectory Analysis.- Treating immune-mediated thrombotic
thrombocytopenic purpura by Caplacizumab.- Treating Immune Thrombocytopenic
Purpura by Immunosuppressive Agents.
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