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E-raamat: Affinity Chromatography: Methods and Protocols

  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2466
  • Ilmumisaeg: 18-May-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071621769
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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2466
  • Ilmumisaeg: 18-May-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071621769
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This volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.

Antibody Purification Using Affinity Chromatography.- TIM4-affinity
Methods Targeting Phosphatidylserine for Isolation or Detection of
Extracellular Vesicles.- Application of Affitins for Affinity Purification of
Proteins.- Identification of Clusterin as a major ABri- and ADan-binding
protein using affinity Chromatography.- Application of a Novel CL7/Im7
Affinity System in Purification of Complex and Pharmaceutical
Proteins.- Positive and Negative Affinity Chromatography to Purify Norovirus
P-Domain Protein.- Robotic Affinity Purification of Soluble and Insoluble
Recombinant Glutathione-S-Transferase Fusion Proteins.- Application of
Immunoaffinity-Mass Spectrometry (IA-MS) for Protein Biomarker
Quantification.- Cellular Membrane Affinity Chromatography for the
Identification of Bioactive Molecules from Complex Natural Mixtures.- Rapid
Affinity Purification of Tagged Plant Mitochondria
(Mito-AP).- Arginine-affinity Chromatographyfor Nucleic Acids (DNA and RNA)
Isolation.- Purification and Analysis of Nucleotides and Nucleosides from
Plants.- Ligand Selection for Affinity Chromatography Using Phage Display.-
Selection and Application of Aptamer Affinity for Protein Purification.-
Entrapment of Proteins within Columns for High-Performance Affinity
Chromatography.- Preparation of Affinity Chromatography Monolith in
Miniaturized Format and Application for Protein Separation.- Ligand
Immobilization Methods for Affinity Chromatography.- Preparation of
Molecularly Imprinted poly(N-isopropylacrylamide) Thermosensitive Based
Cryogels.- Preparation of Staphylococcal Protein a Imprinted Supermacroporous
Cryogel Beads.