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E-raamat: African Swine Fever Virus: Methods and Protocols

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African Swine Fever Virus: Methods and ProtocolsSuurem pilt
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This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.  

Working safely with African swine fever virus.- Necropsy procedures and
evaluation of macroscopic lesions of pigs infected with African swine fever
virus.- Preparation of immunofluorescently labelled tissue sections for
imaging at low and high magnifications in the confocal microscope.- Primary
macrophage culture from porcine blood and lungs.- Isolation of porcine bone
marrow cells and generation of recombinant African swine fever
viruses.- Laboratory diagnosis and quantification of African swine fever
virus using real-time polymerase chain reaction.- Quantification of ASFV DNA
and RNA in Ornithodoros soft ticks.- Genotyping of African swine fever
virus.- African swine fever virus (ASFV) indirect ELISA test based on the use
of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag).- Indirect
immunoperoxidase test (IPT) for detection of antibodies against African swine
fever virus (ASFV) on African green monkey cell lines (Vero, MS).- African
Swine Fever Virus Haemadosrption Inhibition Assay.- ELISpot assay for the
detection of ASFV-specific interferon-gamma (IFN-) producing
cells.- Purification of African swine fever virus.- African swine fever virus
plaque assay and disinfectant testing.- Bioorthogonal labelling of African
swine fever virus infected cells.- Whole genome sequencing of African swine
fever virus.
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