Biocatalyst Immobilization: Foundations and Applications provides a comprehensive overview of biocatalytic immobilization processes, as well as methods for study, characterization and application. Early chapters discuss current progress in enzyme immobilization and methods for selecting and pretreating enzymes prior to immobilization, with an emphasis on navigating common challenges and employing enzyme supports and post immobilization treatments to impact enzymatic activity. Process-based chapters instruct on measuring and reporting on enzyme immobilization efficiency, protein final content, quantification of reaction products, and the use of nanomaterials to characterize immobilized enzymes.
Later chapters examine recent advances, including novel enzymatic reactors, multi-enzymatic biocatalysts, enzymatic biosensors, whole cell immobilization, the industrial application of immobilized enzymes, and perspectives on future trends.
- Provides a thorough overview of biocatalyst and enzyme immobilization for research and practical application
- Presents methods based content that instructs in enzyme immobilization pretreatment, enzyme supports, post immobilization treatments, measuring enzyme immobilization efficiency, quantification of reaction products, and whole cell immobilization
- Features chapter contributions from international leaders in the field
CHAPTER 1 The enzyme, the support, and the immobilization strategy: The
key findings to a desirable biocatalyst
Karen Rodrguez-Nunez, Claudia Bernal, and Juan M. Bol´var
CHAPTER 2 Selection and modification of enzymes prior to immobilization
Ja Hyun Lee, Hah Young Yoo, and Seung Wook Kim
CHAPTER 3 Various approaches to enzyme immobilization
Syed Awais Attique, Qurat ul ain, Nazim Hussain, Muhammad Bilal, and Hafiz
M.N. Iqbal
CHAPTER 4 Postimmobilization treatments before applications
Karolina Labus and Jolanta Bryjak
CHAPTER 5 Support-free immobilization
Roberta Bussons Rodrigues Val
CHAPTER 6 Measuring and reporting enzyme´s immobilization efficiency
Paula Nicolas, Carla Jos
CHAPTER 7 Some recent innovations related to enzyme immobilization
Bhaskar Das, Satyam, and Sanjukta Patra
CHAPTER 8 Enzyme immobilization for use in nonconventional media
Mara Laura Foresti and Mara Lujan Ferreira
CHAPTER 9 Assaying the biological activities of immobilized enzymes
Daniel Sanchez and Mara Lujan Ferreira
CHAPTER 10 Enzyme immobilization on nanomaterials and nanostructured
supports
Azeem Intisar, Mateen Haider, Muhammad Imran Din, Nazim Hussain, Muhammad
Bilal, and Hafiz M.N. Iqbal
CHAPTER 11 Oriented immobilization of biomolecules on small surfaces
Masumi Iijima and Shunichi Kuroda
CHAPTER 12 Affinity immobilization and affinity layers
Meryam Sardar and Kainat Mirza
CHAPTER 13 New enzymatic reactor designs: From enzymatic batch to 3D
microreactors and monoliths
Kim Shortall, Katarzyna Szymanska, Cristina Carucci, Tewfik Soulimane, and
Edmond Magner
CHAPTER 14 Immobilization of multienzymes: Problems and solutions
Yingjie Du, Le Zhong, Ziyuan Wang, Yuxiao Feng, Jiandong Cui, and Shiru Jia
CHAPTER 15 An introduction to enzymatic biosensors
Tayyaba Anjum, Nazim Hussain, Muhammad Bilal, and Hafiz M.N. Iqbal
CHAPTER 16 Industrial applications of immobilized enzymes: Food and beyond
Mara Lujan Ferreira
CHAPTER 17 Future perspectives in enzyme immobilization
Lorena Wilson, Andr
PhD in Chemistry María Luján Ferreira is a Principal Investigator at CONICET (NATIONAL COUNCIL OF SCIENTIFIC AND TECHNICAL RESEARCH) of Argentina. She has published more than 150 articles in peer reviewed, international journals, and has participated with presentations in more than 80 argentinian scientific events and more than 120 international scientific meetings. Dr. Ferreira has also directed three Assistant Investigators at CONICET. Her research focuses on all practical aspects of enzyme immobilization (including applied biotechnology) and the details of the experimental design of enzymatic reactions: how to prepare immobilized biocatalysts and sampling in biocatalytic reactions ( from biphasic and multiphasic reaction media) avoiding mistakes and optimizing procedures. Her interests include the molecular modeling of biocatalysts. In more than 20 years of scientific career she has studied polymerization catalysts, heterogeneous metallic catalysts and recently also nanozymes and ionzymes applied in environmental remediation (of dyes and pharmaceuticals) using advanced oxidation processes.
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