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E-raamat: Biological Microarrays: Methods and Protocols

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Recent developments in microarray technology have changed the landscape of biology and biomedical research, and they have revolutionized RNA and DNA research. In Biological Microarrays: Methods and Protocols, expert researchers explore exciting new developments in the field, providing a comprehensive approach to biological microarrays that conveys not only the state-of-the-art fundamentals, but also includes applications of the most innovative methods. Chapters address both the application of biological microarrays, including DNA/RNA, apatmer, proteins, tissues, oligonucleotides, carbohydrates, biomaterials, cells, bacteria, and virus microarrays, and also explore the different techniques used for generating microarray platforms. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and revolutionary, Biological Microarrays: Methods and Protocols serves as a primary source for academics, practitioners, and professionals in related fields, including biologists, biotechnologists, biochemists, analytical chemists, and biomedical, physical, and microsystems engineers, to name a few, appealing to all of those interested in the present and future state of biological microarray research.
Preface v
Contributors ix
PART I APPLICATION OF BIOLOGICAL MICROARRAY
1 RNA and DNA Microarrays
3(32)
Stuart C. Sealfon
Tearina T. Chu
2 Aptamer Arrays
35(20)
Eva Baldrich
3 Oligonucleotide Microarrays for Identification of Microbial Pathogens and Detection of Their Virulence-Associated or Drug-Resistance Determinants
55(40)
Dmitriy V. Volokhov
Hyesuk Kong
Keith Herold
Vladimir E. Chizhikov
Avraham Rasooly
4 Protein Microarrays Printed from DNA Microarrays
95(12)
Oda Stoevesandt
Mingyue He
Michael J. Taussig
5 Lithographically Defined Two- and Three-Dimensional Tissue Microarrays
107(10)
Esther W. Gomez
Celeste M. Nelson
6 Ratiometric Lectin Microarray Analysis of the Mammalian Cell Surface Glycome
117(16)
Ku-Lung Hsu
Kanoelani Pilobello
Lakshmipriya Krishnamoorthy
Lara K. Mahal
7 Cell Microarrays Based on Hydrogel Microstructures for the Application to Cell-Based Biosensor
133(14)
Won-Gun Koh
8 Fabrication of Bacteria and Virus Microarrays Based on Polymeric Capillary Force Lithography
147(14)
Pil J. Yoo
9 3D Polymer Scaffold Arrays
161(16)
Carl G. Simon Jr.
Yanyin Yang
Shauna M. Dorsey
Murugan Ramalingam
Kaushik Chatterjee
PART II METHODS FOR MICROARRAY GENERATION
10 PDMS Microfluidic Capillary Systems for Patterning Proteins on Surfaces and Performing Miniaturized Immunoassays
177(18)
Mateu Pla-Roca
David Juncker
11 Merging Photolithography and Robotic Protein Printing to Create Cellular Microarrays
195(12)
Ji Youn Lee
Alexander Revzin
12 Generation of Protein and Cell Microarrays on Functionalized Surfaces
207(12)
Yoo Seong Choi
Chang-Soo Lee
13 Microprinting of Liver Micro-organ for Drug Metabolism Study
219(20)
Robert C. Chang
Kamal Emami
Antony Jeevarajan
Honglu Wu
Wei Sun
14 Microcontact Printing
239(10)
Yunyan Xie
Xingyu Jiang
15 Micromolding for the Fabrication of Biological Microarrays
249(12)
Ashley L. Galloway
Andrew Murphy
Jason P. Rolland
Kevin P. Herlihy
Robby A. Petros
Mary E. Napier
Joseph M. DeSimone
16 Progress Report on Microstructured Surfaces Based on Chemical Vapor Deposition
261(20)
Yaseen Elkasabi
Joerg Lahann
17 Methods for Forming Human Microvascular Tubes In Vitro and Measuring Their Macromolecular Permeability
281(14)
Gavrielle M. Price
Joe Tien
18 Microarray Bioinformatics
295(26)
Robert P. Loewe
Peter J. Nelson
Index 321