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E-raamat: Borrelia burgdorferi: Methods and Protocols

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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2742
  • Ilmumisaeg: 02-Jan-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071635612
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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2742
  • Ilmumisaeg: 02-Jan-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071635612

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This volume covers the latest advancements and techniques used to understand the fastidious bacterium, Borrelia burgdorferi, and its significance in infectious disorders by combining both conventional and cutting-edge approaches. This book covers diverse topics, including direct detection, diagnostic methods, immune response analysis, alternative model systems, advanced proteomics, social media analysis, and clinical research. It also discusses unconventional wet lab research such as content analysis, the use of ChatGPT, clinical algorithms for chronic Lyme, establishment of a pregnancy Lyme disease biobank, and investigates Lyme in pregnant women. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Borrelia burgdorferi: Methods and Protocols encompasses a wide range of techniques and caters to scientists from various disciplines and career stages, such as cell and molecular biologists, statisticians, and clinical researchers. 

 Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or
96 Tick-Borne Pathogens in 48 or 96 Samples.- Direct Detection of Borrelia
Species in Tissues.- Purification of Borrelia burgdorferi Outer Membrane
Vesicles.- Constructing an ELISA for Detection of Anti-Borrelia in Wildlife
and Agricultural Animals.- A Step-by-Step Guide to Selecting an Optimal
Cut-Off Value Based on the Receiver Operating Characteristic and Youden Index
in Methods Designed to Diagnose Lyme Disease.- Adaptive Immune Response
Investigation in Lyme borreliosis.- CYTOSPOT: Intracellular Staining and
Detection of Cytokines by Flow Cytometer, An Alternative to LYMESPOT.- Use of
Specific Borrelia Phages as a New Strategy for Improved Diagnostic
Tests.- Identification of Unambiguous Borrelia Peptides in Human Urine Using
Affinity Capture and Mass Spectrometry.- Preparation of Borrelia Infected
Mammalian Cells for Helium Ion Microscopy.- Establishing a Zebrafish Model
for Borrelia Burgdorferi Infection Using Immersion and Microinjection
Methods.- Development of Organoids to Study Infectious Host
Interactions.- Content Analysis: An Approach to Exploring the Depiction of
Tick-Borne Diseases.- Applying BERT and ChatGPT for Sentiment Analysis of
Lyme Disease in Scientific Literature.- Building a Binary Classification
Machine Learning Model: A Guide to Predicting Participation in a Lyme Disease
Program at a Medical Institute.- Clinical Algorithm and Diagnostic Tools for
Lyme Disease.- Establishing a Pregnancy Lyme Disease Biobank.- Developing a
Prospective Gestational Lyme Disease Study.