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E-raamat: Brain Development: Methods and Protocols

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Brain Development: Methods and ProtocolsSuurem pilt
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This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain.
Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor
Adult Neurogenesis in Hydra.- Reverse Genetic Approaches to Investigate the
Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis.- Generating
Transgenic Reporter Lines for Studying Nervous System Development in the
Cnidarian Nematostella vectensis.- Immunostaining and In Situ Hybridization
of the Developing Acoel Nervous System.- Immunostaining of the Embryonic and
Larval Drosophila Brain.- Non-Fluorescent RNA In Situ Hybridization Combined
with Antibody Staining to Visualize Multiple Gene Expression Patterns in the
Embryonic Brain of Drosophila.- Analysis of Complete Neuroblast Cell Lineages
in the Drosophila Embryonic Brain via DiI Labeling.- Flybow to Dissect
Circuit Assembly in the Drosophila Brain: An Update.- Live Cell Imaging of
Neural Stem Cells in the Drosophila Larval Brain.- CRISPR/Cas9 Genome Editing
to Study Nervous System Development in Drosophila.- The Red Flour Beetle as
Model for Comparative Neural Development: Genome Editing to Mark Neural Cells
in Tribolium Brain Development.- A Protocol for Double Fluorescent In Situ
Hybridization and Immunohistochemistry for the Study of Embryonic Brain
Development in Tribolium castaneum.- Immunohistochemistry and Fluorescent
Whole Mount RNA In Situ Hybridization in Larval and Adult Brains
of Tribolium.- X-Ray Microscopy of the Larval Crustacean Brain.-
Immunolocalization of Neurotransmitters and Neuromodulators in the Developing
Crayfish Brain.- Immunostainings in Nervous System Development of the
Nematode C. elegans.- Methods in Brain Development of Molluscs.- A Simple
Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages
Following In Situ Hybridization.- Spawning Induction and Embryo
Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum.- In
Situ Hybridization and Immunostaining of Xenopus Brain.-Morpholino Studies in
Xenopus Brain Development.- Sensitive Multiplexed Fluorescent In Situ
Hybridization Using Enhanced Tyramide Signal Amplification and Its
Combination with Immunofluorescent Protein Visualization in Zebrafish.- Live
Morphometric Classification of Sensory Neurons in Larval Zebrafish.-
Immunohistochemistry and In Situ Hybridization in the Developing Chicken
Brain.- Gene Silencing in Chicken Brain Development.- Transplantation of
Neural Tissue: Quail-Chick Chimeras.- Immunohistochemistry and RNA In Situ
Hybridization in Mouse Brain Development.- The Cre/Lox System to Assess the
Development of the Mouse Brain.- In Utero Electroporation to Study Mouse
Brain Development.
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