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E-raamat: Cell-Wide Identification of Metabolite-Protein Interactions

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  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2554
  • Ilmumisaeg: 30-Sep-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071626245
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Cell-Wide Identification of Metabolite-Protein InteractionsSuurem pilt
  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2554
  • Ilmumisaeg: 30-Sep-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071626245
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This thorough volume explores protocols of proteome- and metabolome-wide strategies for the identification of protein-small molecule complexes in different organisms, in order to shed light on these important regulatory interactions. Experimental and computational strategies to characterize protein-metabolite interactions are discussed, and recent advances in enabling technologies are featured as well. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice to ensure success in future research. 

Authoritative and practical, Cell-Wide Identification of Metabolite-Protein Interactions will aid researchers seeking a better understanding of the mechanisms of signal transduction occurring in the cell and assessing the effect of complex formation on cell physiology.
Protein-Metabolite Interactions Shape Cellular Metabolism and
Physiology.- Affinity Purification Protocol Starting with a Small Molecule as
Bait.- Cellular Thermal Shift Assay for the Detection of Small
Molecule-Target Interactions in Arabidopsis Cells.- Solvent-Induced Protein
Precipitation for Drug Target Discovery.- Identification of Plant
Protein-Metabolite Interactions by Limited Proteolysis-Coupled Mass
Spectrometry (LiP-MS).- Limited Proteolysis-Mass Spectrometry to Identify
Metabolite-Protein Interactions.- Proteome Integral Solubility Alteration
(PISA) for High-Throughput Ligand Target Deconvolution with Increased
Statistical Significance and Reduced Sample Amount.- Parallel Analysis of
ProteinProtein and ProteinMetabolite Complexes Using a Single-Step Affinity
Purification.- Detecting and Characterizing Interactions of Metabolites with
Proteins by Saturation Transfer Difference Nuclear Magnetic Resonance
(STD-NMR) Spectroscopy.- PROMIS: Co-Fractionation Mass Spectrometry for
Analysis of Protein-Metabolite Interactions.- Metabolomics: Going Deeper,
Going Broader, Going Further.- Specifics of Metabolite-Protein Interactions
and Their Computational Analysis and Prediction.- Biophysical Approaches for
the Characterization of Protein-Metabolite Interactions.- Databases and Tools
to Investigate Protein-Metabolite Interactions.
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