| Contributors |
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xi | |
| Preface |
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xv | |
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1 Fluorogenic detection of protein aggregates in live cells using the AggTag method |
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1 | (22) |
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2 | (5) |
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7 | (3) |
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10 | (13) |
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19 | (1) |
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19 | (4) |
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2 Synthesis and application of a ratiometric probe for hydrogen peroxide |
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23 | (14) |
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24 | (1) |
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25 | (4) |
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3 In vitro detection of hydrogen peroxide |
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29 | (3) |
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4 Ratiometric imaging of hydrogen peroxide accumulation in living cells |
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32 | (3) |
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5 Summary and conclusions |
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35 | (2) |
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35 | (1) |
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35 | (2) |
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3 Protein labeling for FRET with methoxycoumarin and acridonylalanine |
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37 | (34) |
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38 | (3) |
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41 | (4) |
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45 | (5) |
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4 Preparation of a-synuclein (aS) constructs for intramolecular FRET |
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50 | (9) |
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5 Preparation of calmodulin (CaM) constructs for intramolecular and intermolecular FRET |
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59 | (2) |
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6 Fluorescence experiments and FRET calculations |
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61 | (6) |
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67 | (4) |
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67 | (4) |
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4 Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assays |
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71 | (20) |
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72 | (2) |
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2 Fluorescence based-assay for activity and inhibition of ADA |
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74 | (6) |
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3 Discovery assay for inhibitors of ADA |
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80 | (6) |
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86 | (1) |
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5 Analysis and statistics |
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86 | (1) |
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86 | (1) |
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87 | (1) |
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8 Alternative methods/procedures |
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87 | (1) |
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9 Troubleshooting and optimization |
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88 | (1) |
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88 | (3) |
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88 | (1) |
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88 | (3) |
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5 Method to discriminate amyloids using fluorescent probes |
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91 | (24) |
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92 | (2) |
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94 | (18) |
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112 | (3) |
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113 | (1) |
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113 | (2) |
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6 Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives |
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115 | (26) |
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Sujeewa S. Lellupitiyage Don |
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116 | (1) |
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2 Chemically modulating circadian rhythms and cellular behavior using small molecules |
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117 | (1) |
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3 Recording circadian oscillations in cells with real-time luminescence |
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118 | (2) |
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4 Analyzing circadian rhythms in bioluminescence recordings |
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120 | (13) |
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5 Evaluating cellular characteristics |
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133 | (3) |
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136 | (5) |
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136 | (1) |
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137 | (4) |
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7 Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancer |
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141 | (26) |
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142 | (3) |
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145 | (1) |
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145 | (4) |
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149 | (1) |
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149 | (1) |
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150 | (4) |
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7 Dissection and ex vivo imaging of tumors and healthy tissues |
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154 | (1) |
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8 Tissue processing and fluorescence scanning of sections |
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155 | (1) |
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9 Safety considerations and standards |
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156 | (1) |
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10 Analysis and statistics |
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156 | (7) |
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11 Troubleshooting and optimization |
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163 | (1) |
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164 | (3) |
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164 | (3) |
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8 Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles |
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167 | (24) |
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168 | (1) |
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2 Developing and characterizing Qui-based abFP |
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169 | (7) |
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3 Expression of abFP in mammalian cells to sense acidic phi |
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176 | (2) |
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4 Using abFP to fluorescently image receptor endocytosis in mammalian cells |
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178 | (2) |
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180 | (6) |
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186 | (5) |
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186 | (1) |
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186 | (5) |
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9 4-Cyanoindole-based fluorophores for biological spectroscopy and microscopy |
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191 | (26) |
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192 | (5) |
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2 Synthesis of Fmoc-protected 4CN-Trp and incorporation of 4CN-Trp into peptides |
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197 | (5) |
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202 | (9) |
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211 | (6) |
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211 | (1) |
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211 | (6) |
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10 Dissecting contributions of catalytic and reader domains in regulation of histone demethylation |
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217 | (20) |
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218 | (1) |
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2 Preparation of recombinant KDM5A1, 797 |
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219 | (7) |
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3 Assaying peptide binding to PHD1 reader domain of KDM5A |
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226 | (5) |
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4 Assaying the catalytic activity of purified KDM5AW97 |
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231 | (6) |
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235 | (1) |
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235 | (2) |
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11 Ortno-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization |
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237 | (26) |
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238 | (2) |
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2 OPA-Amine reaction for peptide and protein labeling |
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240 | (9) |
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3 OPA-amine-thiol reaction for chemoseletive peptide cyclization |
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249 | (6) |
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255 | (3) |
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258 | (5) |
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259 | (1) |
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259 | (2) |
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261 | (2) |
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12 Chemical synthesis of di-ubiquitin modified histones for further biochemical studies |
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263 | (26) |
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264 | (2) |
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2 Total chemical synthesis of site-specifically K27-diubiquitin modified histones |
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266 | (9) |
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3 Thiirane-directed chemical synthesis of di-ubiquitinated H2A mimics |
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275 | (7) |
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282 | (1) |
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283 | (6) |
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284 | (1) |
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284 | (5) |
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13 Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies |
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289 | (24) |
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290 | (2) |
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2 Functional and biochemical characteristics of the epichaperome |
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292 | (2) |
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3 Epichaperome probe preparation |
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294 | (6) |
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4 Epichaperome detection and quantification |
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300 | (6) |
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5 Data acquisition and analysis |
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306 | (3) |
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309 | (4) |
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309 | (1) |
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309 | (1) |
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310 | (3) |
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14 FIICk (fluorescent isoindole crosslinking) for peptide stapling |
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313 | (20) |
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314 | (9) |
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323 | (1) |
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324 | (1) |
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325 | (1) |
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5 Modifications for bicyclic alpha amanitin analogue |
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326 | (1) |
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6 Preparation of modified ortho-phthalaldehydes |
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327 | (2) |
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329 | (1) |
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330 | (3) |
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331 | (2) |
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15 Selective protein N-terminal labeling with N-hydroxysuccinimide esters |
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333 | (22) |
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334 | (2) |
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2 Native chemical ligation and expressed protein ligation |
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336 | (3) |
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3 N-hydroxysuccinimide esters in protein labeling |
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339 | (3) |
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4 Protocol for N-terminal labeling with N-hydroxysuccinimide esters |
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342 | (2) |
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5 Case study with USP7 labeling with biotin-PEG4-NHS |
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344 | (2) |
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6 Sulfo-cyanine5-NHS labeling of WWP2 |
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346 | (2) |
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7 Summary and future directions |
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348 | (7) |
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348 | (1) |
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348 | (7) |
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16 Covalent live-cell labeling of proteins using a photoreactive fluorogen |
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355 | (24) |
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356 | (4) |
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2 Chemical synthesis of malachite green diazirine |
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360 | (7) |
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3 Preparation of FAP fusion vectors |
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367 | (3) |
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4 Live cell imaging with malachite green diazirine |
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370 | (6) |
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376 | (3) |
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376 | (3) |
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17 Photoconvertible diazaxanthilidene dyes for live cell imaging |
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379 | (10) |
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380 | (2) |
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2 Cell imaging of a photoconvertible dye |
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382 | (4) |
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386 | (1) |
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4 Summary and conclusions |
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387 | (2) |
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387 | (2) |
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18 No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag |
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389 | |
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390 | (2) |
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2 Approach to no-wash site-selective labeling |
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392 | (4) |
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3 hCRBPII-based photoswitchable no-wash NIR fluorescence cell labeling |
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396 | (10) |
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406 | |
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406 | (1) |
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407 | |
Lisainfo e-raamatute kohta