| Contributors |
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xi | |
| Preface |
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xv | |
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1 Fluorescent detection of peroxynitrite during antibody-dependent cellular phagocytosis |
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1 | (36) |
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2 | (6) |
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2 Synthesis of peroxynitrite sensor 3 (PS3) |
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8 | (7) |
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3 Photophysical properties, reactivity, and selectivity of PS3 |
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15 | (6) |
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4 Detection of peroxynitrite resulting from antibody-dependent cellular phagocytosis (ADCP) of opsonized tentagel microspheres |
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21 | (9) |
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30 | (1) |
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30 | (1) |
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31 | (6) |
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2 Synthesis, evaluation, and biological applications of visible-light-controllable nitric oxide releasers |
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37 | (26) |
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38 | (1) |
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39 | (10) |
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3 Protocols: ESR spin trapping method with iron-dithiocarbamate complex |
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49 | (2) |
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4 Protocols: NO electrode |
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51 | (2) |
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5 Protocols: NO detection by fluorescence probes |
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53 | (3) |
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6 Protocols: Determination of quantum yield |
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56 | (2) |
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7 Protocols: Photocontrol of vasodilation using rat aorta strip |
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58 | (2) |
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60 | (1) |
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60 | (1) |
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60 | (3) |
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3 Design of fluorescent protein-based sensors through a general protection-deprotection strategy |
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63 | (20) |
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Gloricelly M. Roman-Arocho |
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64 | (1) |
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2 Site-specific incorporation of noncanonical amino acids into proteins |
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65 | (6) |
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3 A fluorescent protein-based mercury (li) sensor |
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71 | (6) |
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77 | (2) |
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79 | (1) |
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79 | (4) |
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4 Chemiluminescent sensors for quantitation of the bacterial second messenger cyclic di-GMP |
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83 | (22) |
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84 | (3) |
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2 Biosensor expression and purification |
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87 | (8) |
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3 Generating a standard curve for quantitating c-di-GMP |
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95 | (4) |
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4 Bacterial cell extract analysis |
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99 | (4) |
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103 | (1) |
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103 | (2) |
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5 Calcium-responsive liposomes: Toward ion-mediated targeted drug delivery |
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105 | (26) |
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106 | (3) |
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2 Synthesis of calcium-responsive lipid 1 |
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109 | (7) |
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3 Liposome preparation (three types of samples, with encapsulated Nile red, sulforhodamine B and without dye) |
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116 | (3) |
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119 | (2) |
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121 | (1) |
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6 Nile red release with other metal ions |
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122 | (1) |
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123 | (2) |
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125 | (2) |
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9 Summary and conclusions |
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127 | (1) |
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127 | (1) |
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127 | (4) |
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6 Far-red photoactivatable BODIPYs for the super-resolution imaging of live cells |
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131 | (18) |
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132 | (2) |
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2 Equipment and materials |
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134 | (6) |
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140 | (2) |
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142 | (3) |
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145 | (1) |
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6 Summary and conclusions |
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146 | (1) |
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146 | (1) |
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146 | (3) |
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7 Washington Red (WR) dyes and their imaging applications |
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149 | (16) |
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149 | (2) |
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2 Rational design and chemical structures of WR dyes |
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151 | (1) |
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3 PhotophysicaI properties of WR dyes |
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152 | (2) |
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4 Applications of WR dyes in developing NIR probes to image H2S in cells |
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154 | (7) |
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161 | (1) |
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162 | (1) |
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162 | (3) |
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8 Bioluminescence imaging in mice with synthetic luciferin analogues |
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165 | (20) |
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166 | (4) |
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2 General considerations for in vivo imaging with synthetic luciferins |
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170 | (2) |
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3 Imaging of transgenic luciferase mice |
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172 | (3) |
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4 Imaging using adeno-associated viral (AAV) reporters |
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175 | (3) |
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5 Considerations for marine luciferases and luciferins |
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178 | (1) |
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179 | (1) |
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179 | (1) |
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179 | (6) |
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9 Monitoring neuronal activity with voltage-sensitive fluorophores |
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185 | (20) |
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186 | (1) |
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2 Voltage imaging with voltage-sensitive fluorophores |
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187 | (4) |
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3 Primary hippocampal cell culture |
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191 | (3) |
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4 Functional voltage imaging of cultured neurons |
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194 | (5) |
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5 Analysis and interpretation of functional imaging data |
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199 | (1) |
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200 | (1) |
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201 | (1) |
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202 | (1) |
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202 | (3) |
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10 Temporally gated molecular tools for tracking protein-protein interactions in live cells |
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205 | (20) |
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206 | (1) |
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2 SPARK for detecting protein-protein interactions in live cells |
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207 | (3) |
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3 Applying SPARK to detect agonist induced B2AR-GS mimic and B2AR-arrestin interaction |
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210 | (4) |
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214 | (8) |
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222 | (1) |
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222 | (1) |
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222 | (3) |
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11 trLRET microscopy: Ultrasensitive imaging of lanthanide luminophores |
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225 | (24) |
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226 | (2) |
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2 Conceptual basis for trLRET imaging |
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228 | (3) |
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231 | (15) |
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246 | (1) |
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247 | (1) |
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247 | (2) |
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12 Long fluorescence lifetime triangulenium dyes in imaging and fluorescence polarization assay |
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249 | (18) |
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250 | (5) |
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2 Labeling of proteins with reactive triangulenium dyes |
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255 | (1) |
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256 | (1) |
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4 Analysis of the bioconjugate (and complex) |
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257 | (5) |
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262 | (1) |
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262 | (1) |
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262 | (1) |
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263 | (4) |
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13 Didactic approach recounting advances and limitations in novel glutathione and cysteine detection (reduced GSH probe) with mixed coumarin, aldehyde, and phenyl-selenium chemistry |
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267 | (24) |
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269 | (2) |
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2 Protocols and a discussion of materials, equipment, and reagents |
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271 | (12) |
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3 Remaining questions, concerns, caveats |
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283 | (2) |
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4 Conclusions and future outlook |
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285 | (1) |
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286 | (1) |
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286 | (1) |
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286 | (3) |
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289 | (2) |
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14 Detection of cell-surface phosphatidylserine using the fluorogenic probe P-IID |
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291 | (18) |
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292 | (2) |
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2 Principle of the detection of apoptotic cells with P-IID |
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294 | (1) |
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294 | (3) |
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297 | (2) |
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299 | (7) |
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306 | (1) |
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306 | (1) |
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306 | (3) |
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15 Quinoline-based fluorescent small molecules for live cell imaging |
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309 | |
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310 | (3) |
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313 | (7) |
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3 Plate reader analysis of DMAQ derivatives |
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320 | (2) |
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4 Cell imaging of an environmentally sensitive dye |
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322 | (2) |
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5 Summary and conclusions |
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324 | (1) |
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325 | |
Lisainfo e-raamatute kohta