Correlative Light and Electron Microscopy V, Volume 187 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.
1. How to Apply the Broad Toolbox of Correlative Light and Electron
Microscopy to Address a Specific Biological Question
Thomas Müller-Reichert, Erin M. Tranfield, Gunar Fabig and Thomas Kurth
2. Some tips and tricks for a correlative light electron microscopy
workflow using stable expression of fluorescent proteins
Paul Verkade
3. Targeting membrane receptors with fluoronanogold probes for high
resolution correlative microscopy.
Monica Fernandez Monreal
4. Correlative light and electron microscopy at defined cell cycle stages in
a controlled environment
Shotaro Otsuka
5. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) culture and
sample preparation for correlative light electron microscopy
Paul Verkade, Maximillian Erdmann, Isobel Web, Lorna Hodgson and Andrew
Davidson
6. Tissue CLEM
Christine Longin
7. Array tomography of in vivo labeled synaptic receptors
Christian Stigloher and Sebastian Britz
8. Correlative cryo-microscopy pipelines for in situ cellular studies
Anna Sartori and Chiara Zurzolo
9. Building a super-resolution fluorescence cryomicroscope
Thom Sharp
10. Analysis of super resolution CLEM data
Thom Sharp
11. Nanometer targeting using cryo super resolution
Laura Carolina Zanetti-Domingues
12. Laboratory Based Correlative Cryo-Soft X-Ray Tomography and
Cryo-Fluorescence Microscopy
Kenneth Fahy
Thomas Müller-Reichert works in the Core Facility Cellular Imaging (CFCI), Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Germany. Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 100 papers and edited 5 books on CLEM (including 4 Volumes of the Methods in Cell Biology series). PV obtained his PhD at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He is actively involved in shaping the future microscopy landscape with roles in the Royal Microscopical Society and BioimagingUK and a current focus on putting volumeEM on the imaging map through community building and the organisation and co-chairing of the 1st Gordon Research Conference on vEM.
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