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E-raamat: Cryopreservation and Freeze-Drying Protocols

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Cryopreservation and Freeze-Drying ProtocolsSuurem pilt
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This book provides detailed protocols for all the latest methodologies used to assure the long-term biostorage of a diverse range of biological materials. Developed in expert laboratories, the protocols have been painstakingly perfected over the years to provide time-tested, step-by-step instructions that ensure robust and reproducible results. Each protocol deals with the preservation of an organelle, cell, or tissue type, and is accessible even to the nonspecialist because of its cookbook approach. Novel protocols can be readily developed with the help of the "hands-on" Notes sections. Cryopreservation and Freeze-Drying Protocols is an indispensable reference work for both the individual researcher, and all those who want to establish or improve biostorage systems in their laboratories. Its applications range from microbial culture collections, botanic gardens, and zoos to animal husbandry, aquaculture, medicine, human fertilization, and cell and molecular biology.

Arvustused

As a source of data on current practice this book has no rival and will undoubtedly prove its worth...The editors...have succeeded [ in] their aim ...to assist those who are setting up biological storage systems and are seeking detailed descriptions of robust methods. - Cryobiology

Overview. Virus Cryopreservation and Storage. Freeze-Drying and
Cryopreservation of Bacteria. Freeze-Drying Yeast. Cryopreservation of
Yeast Cultures. Cryopreservation and Freeze-Drying of Fungi.
Cryopreservation of Pathogenic and Nonpathogenic Free-living Amoebae.
Long-Term Cryopreservation of Thylakoid Membranes. Cryopreservation of
Algae. Cryopreservation of Plant Protoplasts. A Two-Step or Equilibrium
Freezing Procedure for the Cryopreservation of Plant Cell Suspensions.
Vitrification of Plant Cell Suspensions. Cryopreservation of Shoot-Tips and
Meristems. Cryopreservation of Seeds. Cryopreservation of the Sperm of the
Pacific Oyster Crassostrea gigas. Cryopreservation of Fish Spermatozoa.
Cryopreservation of Avian Spermatozoa. Cryopreservation of Animal and Human
Cell Lines. Cryopreservation of Semen from Domestic Livestock.
Cryopreservation of Mammalian Embryos: Slow Cooling. Cryopreservation of
Mammalian Embryos: Vitrification. Cryopreservation of Human Gametes.
Cryopreservation of Human Red Blood Cells. Index.
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