Genomic technologies provide the means of diagnosis and management of many human diseases. Without insights from cytogenetics, correct interpretation of modern high-throughput results is difficult, if not impossible. This book summarizes applications of cytogenetics and molecular cytogenetics for students, clinicians and researchers in genetics, genomics and diagnostics. The book combines the state-of-the-art knowledge and practical expertise from leading researchers and clinicians and provides a comprehensive overview of current medical and research applications of many of these technologies.
KEY FEATURES
Provides clear summaries of fluorescence in situ hybridization technologies and others
Comprehensively covers established and emerging methods
Chapters from an international team of leading researchers
Useful for students, researchers and clinicians
| Series Preface |
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xi | |
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| Preface |
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xiii | |
| Acknowledgements |
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xv | |
| Editor |
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xvii | |
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xix | |
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Chapter 1 Cytogenetics and Chromosomics |
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1 | (6) |
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Chapter 2 Banding Cytogenetics |
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7 | (20) |
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Chapter 3 Generation of Microdissection-Derived Painting Probes from Single Copy Chromosomes |
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27 | (8) |
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Chapter 4 FISH--An Overview |
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35 | (8) |
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Chapter 5 FISH--Microscopy and Evaluation |
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43 | (6) |
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Chapter 6 FISH--in Routine Diagnostic Settings |
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49 | (22) |
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Chapter 7 FISH--in Leukemia Diagnostics |
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71 | (34) |
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Chapter 8 FISH--in Tissues |
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105 | (6) |
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Chapter 9 FISH--in Human Sperm and Infertility |
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111 | (6) |
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Chapter 10 FISH--in Spontaneously Aborted Products of Conception |
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117 | (4) |
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Chapter 11 FISH--Characterization of Chromosomal Alterations, Recombination, and Outcomes after Segregation |
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121 | (30) |
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Chapter 12 Multicolor-FISH--Methods and Applications |
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151 | (6) |
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Chapter 13 FISH--Centromere- and Heterochromatin-Specific Multicolor Probe Sets |
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157 | (6) |
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Chapter 14 FISH--Detection of Individual Radio Sensitivity |
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163 | (8) |
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Chapter 15 FISH--Detection of CNVs |
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171 | (10) |
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Chapter 16 FISH--Interphase Applications Including Detection of Chromosome Instability (CIN) |
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181 | (8) |
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Chapter 17 FISH--Determination of Telomere Length (Q-FISH/CO-FISH) |
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189 | (12) |
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Jelena Filipovic Trickovic |
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Chapter 18 FISH--in Three Dimensions--3D-FISH |
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201 | (6) |
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Chapter 19 FISH--On Fibers |
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207 | (4) |
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Chapter 20 FISH--and Single-Cell Gel Electrophoresis Assay (Comet Assay) |
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211 | (14) |
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Chapter 21 Molecular Karyotyping |
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225 | (26) |
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Chapter 22 FISH--Mitochondrial DNA |
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251 | (12) |
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Chapter 23 FISH--in Birds |
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263 | (18) |
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Michelly da Silva dos Santos |
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Edivaldo Herculano Correa de Oliveira |
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Chapter 24 FISH--in Fish Chromosomes |
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281 | (16) |
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Chapter 25 FISH--and the Characterization of Synaptonemal Complex |
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297 | (10) |
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Chapter 26 RNA-FISH--on Lampbrush Chromosomes: Visualization of Individual Transcription Units |
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307 | (12) |
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Chapter 27 FISH--in Insect Chromosomes |
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319 | (20) |
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Chapter 28 FISH--in Plant Chromosomes |
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339 | (14) |
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Chapter 29 FISH--and CRISPR/CAS9 |
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353 | (4) |
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| Index |
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357 | |
Thomas Liehr is working in human genetics since 1991. He is biologist by education and head of the molecular cytogenetic group in Jena, Germany. Research fields include clinical genetics, leukemia cytogenetics and interphase structure of human chromosomes. The results of his research are published in >10 books, ~100 book chapter, ~800 referred papers, and ~1000 abstracts. His particular expertise includes small supernumerary marker chromosomes (sSMC), chromosomal heteromorphisms and uniparental disomy see ChromosOmics databases (http://cs-tl.de/DB.html). He is active member of the European Board of Medical Genetics and received multiple prices, two invited professorships and a Dr.h.c. as summarized at http://cs-tl.de/.
Lisainfo e-raamatute kohta