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E-raamat: Dengue Virus: Methods and Protocols

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Dengue Virus: Methods and ProtocolsSuurem pilt
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This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by covering protocols to study the interactions between DENV and cellular proteins, DENV immunopathogenesis and diagnosis, as well as recent advances in animal models that can be used in studies of DENV immunopathogenesis and vaccine development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Dengue Virus: Methods and Protocols serves as an ideal guide for researchers attempting to further understand this critical threat to worldwide public health.
Virus Propagation and Plaque Assay for Dengue Virus.- Transmission
Electron Microscopy Studies of Dengue Viruses.- Production of Properly Folded
NS1 Protein in Bacterial Cells.- Expression and Purification of Dengue NS1
Protein in Sf9-Baculovirus System.- Purification of Dengue and Zika Virus
Non-Structural Protein 5 for Crystallization and Screening of Antivirals.-
Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly.-
Construction of Genomic and Sub-Genomic Dengue Infectious Replicons.- Dengue
Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair
Correlation Analysis.- Yeast Two-Hybrid System for Mapping Novel Dengue
Protein Interactions.- Isolation and Identification of Dengue Virus
Interactome with Human Plasma Proteins by Affinity Purification-Mass
Spectrometry.- Molecular Diagnosis Of Dengue.- Serological Diagnosis of
Dengue.- Measuring Transendothelial Electrical Resistance (TEER) for Dengue
Infection Studies.- Evaluation of DENV-InducedEndothelial Cell Permeability
by Measurements of Transendothelial Electrical Resistance (TEER) and
Extravasation of Proteins and Virus.- In Vitro Cytokine Production by
Dengue-Infected Human Monocyte-Derived Dendritic Cells.- In Vitro Generation
of Human Antibody-Secreting Cells through the Stimulation of PBMCs with
Dengue Virus Particles.- Isolation of Microvesicles from Plasma Samples
Avoiding Lipoprotein Contamination.- Evaluating Dengue Virus Pathogenesis in
Mice and Humans by Histological and Immunohistochemistry Approaches.-
Humanized Mice for the Study of Dengue Disease Pathogenesis: Biological
Assays.
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