DNA Damage and Chromosomal Instability, Volume 182 in the Methods in Cell Biology series, presents interesting chapters on a variety of timely topics, including Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation, Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents, Genetic reporters to detect and quantify homologous recombination in yeast, Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variants, Enrichment of DNA replication intermediates by EdU pull down, Nascent DNA sequencing and its diverse applications in genome integrity research, and much more. Additional chapters cover Enrichment of DNA replication intermediates by EdU pull down, DNA curtains for studying phase separation mechanisms of DNA-organizing proteins, Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule Level, Cloning and Expansion of Repetitive DNA Sequences, Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy Arne Lindqvist
Arvustused
"...addresses experimental methods for inducing DNA damage and chromosome instability, methods for evaluating the mechanisms of causation, and approaches to the study of the consequences of these events. It also explores mechanisms of repair of DNA and chromosome damage,... the cumulative work of 55 authors.... [ H]ighly technically focused,... each chapter begins with a short abstract followed by introductory and background information. Detailed laboratory methods follow the introduction, and these include step-by-step instructions for the procedures discussed.... [ U]niquely easy to use for active researchers . Overall, this volume accomplishes what it sets out to do quite well. The detailed laboratory methods presented in each chapter are a strong point of the book." --©Doodys Review Service, 2024, Mark F. Sanders, PhD (UC Davis College of Biological Sciences)
1. Induction of chromosome-specific micronuclei and chromothripsis by
centromere inactivation
Peter Ly
2. Generation of aneuploid cells and assessment of their ability to survive
in presence of chemotherapeutic agents
Stefano Santaguida
3. Genetic reporters to detect and quantify homologous recombination in
yeast
Loraine Symington
4. Mapping histone variant genomic distribution: exploiting SNAP- Tag
labelling to follow the dynamics of incorporation of H3 variants
Geneviève Almouzni and Jean-Pierre Quivy
5. Nascent DNA sequencing and its diverse applications in genome integrity
research
Andre Nussenzweig and Jacob Paiano
6. Enrichment of DNA replication intermediates by EdU pull down
Ylli Doksani
7. DNA curtains for studying phase separation mechanisms of DNA-organizing
proteins
Johannes Stigler
8. Visualizing the Dynamics of DNA Replication and Repair at the
Single-Molecule Molecule Level
Gheorghe Chistol
9. Cloning and Expansion of Repetitive DNA Sequences
Gideon Coster and Sophie Lyn Williams
10. Using an ImageJ-based script to detect replication stress and associated
cell cycle exit from G2 phase by fluorescence microscopy
Arne Lindqvist
11. An automated image analysis pipeline to quantify the coordination and
overlap of transcription and replication activity in mammalian genomes
Stephan Hamperl
12. Monitoring Chk1 kinase activity dynamics in live single cell imaging
assays
Olivier Gavet and Vicente Lebrec
13. Visualising DNA damage and repair using single molecule super resolution
microscopy
Donna Whelan
14. Quantification of nuclear deformations and DNA damage foci dynamics by
live-cell imaging
Francisca Lottersberger
15. Native FISH: A low- and high- throughput assay to analyze the Alternative
Lengthening of Telomere (ALT) pathway
Eros Lazzerini-Denchi and Benura Azeroglu
16. Assessment of DNA Fibres
Chris Staples
17. A high-content flow cytometry and dual CRISPR-Cas9 based platform to
quantify genetic interactions Priyanka Verma
18. Monitoring APOBEC3A protein
levels in human cancer cells
John Maciejowski
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
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