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E-raamat: DNA Damage Responses: Methods and Protocols

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DNA Damage Responses: Methods and ProtocolsSuurem pilt
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This volume provides detailed methods and key approaches used to mechanistically study DNA damage, as well as the factors involved in the damage response. Chapters guide readers through proteomics and biophysical approaches, analyzing protein function, quantifying DNA replication dynamics and nucleic acid base damage, as well as biochemical reconstitution of key pathways involved in DNA repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, DNA Damage Responses: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Robust Computational Approaches to Defining Insights on the Interface of
DNA Repair with Replication and Transcription in Cancer.- A Whole Genome
CRISPR/Cas9 Screening Approach for Identifying Genes Encoding DNA
End-Processing Proteins.- Immunoaffinity Purification of Epitope-Tagged DNA
Repair Complexes from Human Cells.- Universally Accessible Structural Data on
Macromolecular Conformation, Assembly, and Dynamics by Small Angle X-Ray
Scattering for DNA Repair Insights.- Assessing DNA damage responses using B
lymphocyte cultures.- Studying single stranded DNA gaps a­­t replication
intermediates by electron microscopy.- Approaches to monitor termination of
DNA replication using Xenopus egg extracts.- Use of high-performance liquid
chromatography coupled to mass spectrometry to quantify modified
nucleosides.- Targeted Formation of 8-oxoguanine in Telomeres.- Qualitative
and Quantitative Analysis of DNA-Cytidine Deaminase
Activity.- Characterization of DNA-PK-bound end fragments using
GLASS-ChIP.- Monitoring Nuclease Activity by X-ray Scattering Interferometry
using Gold Nanoparticle Conjugated DNA.- In Vitro Reconstitution of
BRCA1-BARD1/RAD51 Mediated Homologous DNA Pairing.- Purification of
DNA-dependent protein kinase catalytic subunit (DNA-PKcs) fromHeLa
cells.- Purification and characterization of human DNA ligase III complexes
after expression in insect cells.- Generation of monoubiquitin and K63-linked
polyubiquitin chains for protein interaction studies.
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