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E-raamat: Euglenozoa: Methods and Protocols, Volume 1

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This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 1 presents a historical review of the research into euglenozoans, followed by sections of techniques involving isolation and culturing of different species of euglenozoans, as well as a variety of -omics research methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.



Authoritative and practical, Euglenozoa: Methods and Protocols, Volume 1 serves as an ideal guide for researchers working with these fascinating organisms and investigating the aspects of their extreme biology.



Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Why Euglenozoans.- Isolation and Cultivation of Diplonemids.-
Preparation of Diplonemid Samples for Microscopy.- Protocol for the Molecular
Identification of Autotrophic Euglenids in Environmental Samples.- Culture
and Genetic Modification of the Monoxenous Trypanosomatids Crithidia
fasciculata and Crithidia bombi.- Poly(A) Profiling for Euglenozoans Using
Oxford Nanopore Direct RNA Sequencing, Illustrated by the Example of Euglena
gracilis.- Euglenid Extrachromosomal DNA: Assembly and Annotation.- Nuclear
Genome Assembly and Annotation of Kinetoplastids.- Assembly and Annotation of
Kinetoplastid and Diplonemid Mitochondrial Genomes.- Cleavage Under Targets
and Release Using Nuclease (CUT&RUN) for Trypanosoma brucei Parasites.-
Mapping Open Chromatin in Trypanosoma brucei Using ATAC-Seq.- Transcriptomic
Analysis of Trypanosomatids.- Small RNA Profiling in Trypanosomes.-
Desorption Electrospray IonizationMass Spectrometry Imaging (DESI-MSI) of
Metabolites in Heart Samples Infected with Trypanosoma cruzi.- Microbiome
Profiling in Chagas Disease: Sample Collection, Sequencing, and Analysis.-
Metabolomics Analysis of Trypanosomes Using Ion Mobility-Enhanced Mass
Spectrometry.- Exploring Protein Interactomes Using TurboID-Directed
Proximity Labeling and Mass Spectrometry.- Mapping Trypanosoma Protein
Interactome by Proximity-Dependent Biotinylation.- From Genome to Function:
Metabolism-Wide Models of Lifecycle Stages of Parasites.- Enhanced Detection
of Homology Using Artificial Intelligence in Euglenids.
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