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E-raamat: Euglenozoa: Methods and Protocols, Volume 2

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This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 2 presents three sections exploring techniques in molecular biology, cell biology, as well as biochemistry and physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.



Authoritative and practical, Euglenozoa: Methods and Protocols, Volume 2 serves as an ideal guide for researchers working with these fascinating organisms and investigating the aspects of their extreme biology.



Chapters 21 and 22 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Genetic Manipulation of Paradiplonema papillatum.- Protocol for
Efficient Ribodepletion of Euglena gracilis RNA.- Engineering Wax Ester
Composition in Euglena gracilis Using Genome Editing.- Multi-Approach
Workflows for Chromatin and Histone Isolation from Trypanosoma cruzi: From
Basic Protein Extraction to Mass Spectrometry-Compatible Protocols.-
Precision Without Selection: A Marker-Free CRISPR/Cas9-Based Protocol for
Multiplexed Genome Editing in Trypanosomatids.- Application of
Double-Inducible Expression System in Bloodstream-Form Trypanosoma brucei.-
Tetracycline-Inducible Functional Complementation Analysis in Trypanosoma
Parasites.- Immunoprecipitation of Protein Complexes from Trypanosoma
brucei.- Standardized Recombinase Polymerase Amplification (RPA) Assay for
Detecting Active Trypanosoma brucei evansi Infections.- High Throughput Cell
Cycle and Morphological Analysis of Leishmania mexicana and Other
Kinetoplastids.- Protein Localization with Streptavidin-Imaging: Boost Your
Signal, Reduce Noise, Overcome Antibody Accessibility Problems, and
Time-Travel to the Past.- A Practical Guide to Ultrastructural Expansion
Microscopy (U-ExM) in Trypanosoma brucei.- Expansion Microscopy in
Euglenozoa.- Colab-PEX19BS: An Automated Google Colab Notebook-Based Tool for
Detecting PEX19 Binding Sites.- Biochemistry of Diplonemids.- Euglena
gracilis Subcellular Fractionation.- Isolation of Mitochondria and
Chloroplasts from Euglena gracilis Z.- Imaging Ca2+ Signaling in Trypanosoma
cruzi with Genetically-Encoded Ca2+ Indicators.- Enzyme Assays for
Phosphoinositide Kinases and Phosphatases and Inhibitor Screens.-
Non-Invasive, Fluorescence-Based Detection of Perturbations in the
Thiol-Redox Homeostasis of Bloodstream Trypanosoma brucei brucei.- Assessment
of Mitochondrial Membrane Potential in Intact and Detergent-Permeabilized
Trypanosoma brucei Insect and Bloodstream Forms.- Camelid Single-Domain
Antibodies as Tools for Novel Target Identification in Kinetoplastid
Research.- Method for Purification and Electrophysiological Recordings of Ion
Channels from Trypanosomatids.- Functional Studies of Kinetoplastid
Transporters.
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