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E-raamat: Experimental Models of Cardiovascular Diseases: Methods and Protocols

  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2803
  • Ilmumisaeg: 27-Apr-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071638460
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  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2803
  • Ilmumisaeg: 27-Apr-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071638460

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This second edition provides new and updated methods for establishing reliable and reproducible experimental models of cardiovascular diseases. Chapters detail practical protocols from expert laboratories focusing on cardiovascular research, that would be critical in exploring novel discoveries in cardiac biology, and the development of effective therapeutic approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.





 





Authoritative and cutting-edge, Experimental Models of Cardiovascular Diseases: Methods and Protocols, Second Edition aims to provide detailed and practical protocols that will be valuable tools for researchers in cardiology to conduct their research.
Decellularized extracellular matrix for modelling cardiac extracellular
microenvironment.- Subtype and lineage- mediated protocol for standardizing
activin/nodal and BMP signaling for hiPSC-derived cardiomyocyte
differentiation.- Generation of 3D-multicellular human iPSC-heart organoids
for the non-invasive assessment of cardiac fibrosis.- In vitro experimental
approach for studying human pulmonary artery smooth muscle cells and
endothelial cells proliferation and migration.- Culturing cardiac tissue
slices under continuous physiological mechanical stretches.- Quantitative
assessment of mitochondrial morphology and electrophysiological function in
the diabetic heart.- Ex vivo working porcine heart model.- Conventional
Method of Cardiac Ischemia/Reperfusion Injury in Mice.- Rat model of
Isoproterenol-induced myocardial injury.- Pop-out Myocardial Infarction
Induction in Mice.- Rat Models of Cardiorenal Syndrome and Methods for
Functional Assessment.- The Sugen/Hypoxia rat model for pulmonary
hypertension and right heart failure.- Unilateral lung removal in combination
with monocrotaline or SU5416 in rodents: a reliable model to mimic the
pathology of the human pulmonary hypertension.- Swine model of myocardial
infarction induced by ischemia-reperfusion and embolization.- Porcine Model
of Hypertrophy-Independent Left Ventricular Stiffening via Repetitive
Pressure Overload.- Swine model of coronary artery
dissection.- Hypoxia-induced piglet model of cardiac arrest with assisted
resuscitation by extracorporeal membrane oxygenation.- A dynamic sheep model
to induce pulmonary hypertension and right ventricular failure.