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E-raamat: Fasciola hepatica: Methods and Protocols

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This volume described basic and advanced protocols to study F. hepatica parasite biology. Chapters guide readers through protocols on different developmental stages of F. hepatica, characterize tegumental, secreted proteins, spatial and temporal gene expression, immunological effects of ESP on macrophages, eosinophil apoptosis, macrophages alternative activation, toll-like receptor interactions, obtain peritoneal and splenic dendritic cells of mice, and protocols to detect F. hepatica albendazole resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Fasciola hepatica: Methods and Protocols provides different topics in areas such a biochemistry, immunology, molecular biology, microscopy, vaccinology in order to extend the interest in this book to research community working with Fasciola hepatica and related trematodes.

Maintenance of Life Cycle at the Laboratory.- Microscopical Techniques
to Analyse the Hepatic and Peritoneal Changes Caused by Fasciola
hepatica Infection.- Isolation of Secreted and Tegumental Surface Proteins
from Fasciola hepatica.- Isolation and Analysis of Fasciola
hepatica Extracellular Vesicles.- Cloning and Heterologous Expression of
Protein-Coding Sequences in Escherichia coli.- Gene Silencing in the Liver
Fluke Fasciola hepatica: RNA Interference.- Analysis of Gene Expression
in Fasciola hepatica Juveniles and Adults by In Situ Hybridization.- Evasion
of Host Immunity during Fasciola hepatica Infection.- Immunomodulatory Effect
of Fasciola hepatica Excretory-secretory Products on Macrophages.- Study of
Eosinophil Apoptosis Induced by Fasciola hepatica Excretory-secretory
Products.- Purification of native Fasciola hepatica Fatty Acid Binding
Protein and the Induction of Alternative Activation of Human Macrophages.-
Possible Role for Toll-Like Receptors in Interaction of Fasciola hepatica
Excretory-Secretory Products with Human Monocyte Cell Line.- Evaluation of
the Immune Regulatory Properties of Dendritic Cells during Fasciola
hepatica Infection.- Design of a Peptidecarrier Vaccine Based on the Highly
Immunogenic Fasciola hepatica Leucine Aminopeptidase.- Liver Fluke Vaccine
Assessment in Cattle.- Testing Albendazole Resistance in Fasciola
hepatica.- Drug Targets: Screening for Small Molecules that Inhibit Fasciola
hepatica Enzymes. 
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