This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications.
Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process.
This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.
Chapter
1. General Principles.
Chapter
2. Signals Scattering.-
Chapter
3. Signals - Fluorescence, Phosphorescence, Impedance, Extinction.-
Chapter
4. Fluidics.
Chapter
5. Light Sources.
Chapter
6. Optical Benches.-
Chapter
7. Detectors and Electronics.
Chapter
8. Signal Analysis.
Chapter
9. The Cytometric File.
Chapter
10. Data Transformation.
Chapter
11. Data
Representation.
Chapter
12. Data Analysis.
Chapter
13. Standards, Setup,
Calibration, and Control Techniques.
Chapter
14. Fluorochromes Overview.-
Chapter
15. Fluorochromes Suitable for Antibody Conjugation.
Chapter
16.
Fluorochromes That Bind Nucleic Acids.
Chapter
17. Fluorochromes for the
Study of the Cell Features.
Chapter
18. Fluorescent Proteins.
Chapter
19.
Spillover and Compensation.
Chapter
20. Artifacts.
Chapter
21. Cell
Sorting.
Chapter
22. Non-Conventional Flow Cytometry.
Chapter
23.
Statistics: A Cytometric Point of View.
Dr. Claudio Ortolani was a senior consultant (now retired) in Clinical Pathology at the Hospital DellAngelo, Venice, Italy.
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