E-raamat: Fluorescence In Situ Hybridization (FISH): Application Guide

Background.- Microscopy and imaging: Visualization matters.- Optical
filters and light sources for FISH.- Automation of FISH-procedure and
evaluation.- Classification of FISH probes.- Commercial FISH probes.-
Generation of painting probes from flow-sorted and microdissected
chromosomes.- FISH-microdissection.- Homemade locus-specific FISH probes:
Bacterial artificial chromosomes.- The standard FISH procedure.- Microwave
treatment for better FISH results in a shorter time.- FISH with and without
COT DNA.- One-day FISH procedure.- Quantitative FISH (Q-FISH).- Telomere
length measurement by FISH: Image processing techniques.- Directional Genomic
Hybridization (dGH) for high-resolution detection of intrachromosomal
rearrangements.- The replicative detargeting FISH (ReD-FISH) technique in
studies of telomere replication.- Pre- and postnatal diagnostics and research
on peripheral blood, bone marrow chorion, amniocytes, and fibroblasts.-
Application of FISH to previously GTG-banded and/or embedded cytogenetic
slides.- FISH in native amniocytes.- Tumorcytogenetic diagnostics and
research on blood and bone marrow smears or effusions.- Characterization of
mosaicism in different easy-to-acquire body tissues such as buccal smears,
skin abrasions, hair root cells or urine.- Characterization of archived
formalin-fixed/paraffin-embedded or cryofixed tissue, including nucleus
extraction.- FISH on sperm, spermatocytes and oocytes.- Fluorescence in situ
hybridization on DNA fibers: Molecular combing.- Two- to three-color FISH.-
Multiplex FISH and spectral karyotyping .- FISH banding techniques.-
Centromere directed M-FISH approaches (cenM-FISH).- Heterochromatin directed
M-FISH (HCM-FISH).- FISH for repeatedly observed euchromatic variants.-
Subtelomeric and/or subcentromeric probe sets.- Bar-coding is more actual
than ever.- FISH for repeatedly observed constitutional rearrangements.-
Parental origin determination FISH: Pod-FISH.- Simultaneous FISH and
fluorescence immunostaining for DNAprotein co-detection.- CENP-antibodies
used additionally to FISH.- X-inactivation accessed by FISH and
EDU-immunostaining.- Immuno-FISH and microspreading technique for
synaptonemal complex studies.- Yeast chromosomes revealed by immuno FISH.-
Interphase FISH in diagnostics.- Interphase FISH for detection of chromosomal
mosaicism and instability.- Comet-FISH.- Micronucleus FISH.-
Three-dimensional interphase analysis using suspension FISH.- 3D DNA-FISH
protocol for the study of the DUX gene in preimplantation bovine embryos.-
Animal probes and Zoo-FISH.- Three-dimensional co-immuno and RNA-fluorescence
in situ hybridization in preimplantation mouse embryos.- FISH in bird
chromosomes: An updated protocol and best practices.- An updated standard
protocol for FISH mapping in fish species (fish-FISH).- FISH in
lampbrush-chromosomes.- General protocol for FISH on insect chromosomes.-
FISH in Drosophila.- FISH on insect cells transfected with heterologous DNA
a single cell directed approach.- Fluorescence in situ hybridization for
plants: Methods of chromosomal preparation.- Mitochondrial DNA (MtDNA)
detection by FISH.- Advances in the application of FISH in food
microbiology.- Evaluation of polymicrobial involvement using Fluorescence In
Situ Hybridization (FISH) in clinical practice.- Comparative genomic
hybridization (CGH) and microdissection-based CGH (micro-CGH) in human.-
Comparative genomic hybridization (CGH) in animals.- Breakpoint mapping of
balanced chromosomal rearrangements using array-CGH with
microdissection-derived FISH probes.- Array CGH.- Chromosome microarray
results to be verified by FISH.- Sequencing of microdissection derived
FISH-probes.- Next generation sequencing should consider molecular
cytogenetic results.- Optical genome mapping results need to be verified by
FISH.- Application of ALFA-tag signal amplification to expand the
CRISPR-based DNA imaging toolkit.