| Preface to First Edition |
|
vii | |
| About the Editor |
|
xiii | |
| Background |
|
1 | (16) |
|
|
|
|
|
Part I Required Equipment and Probes for FISH Procedures |
|
|
|
|
|
17 | (10) |
|
|
|
Optical Filters and Light Sources for FISH |
|
|
27 | (16) |
|
|
|
|
|
|
|
Classification of FISH Probes |
|
|
43 | (6) |
|
|
|
|
|
49 | (14) |
|
|
|
Generation of Paint Probes from Flow-Sorted and Microdissected Chromosomes |
|
|
63 | (18) |
|
|
|
|
|
|
|
|
|
|
|
|
|
81 | (20) |
|
|
|
|
|
|
|
Homemade Locus-Specific FISH Probes: Bacterial Artificial Chromosomes |
|
|
101 | (8) |
|
|
|
|
|
|
The Standard FISH Procedure |
|
|
109 | (10) |
|
|
|
|
|
|
|
|
|
|
|
Microwave Treatment for Better FISH Results in a Shorter Time |
|
|
119 | (4) |
|
|
|
|
|
FISH with and Without COT1 DNA |
|
|
123 | (12) |
|
|
|
|
|
|
|
|
|
Formamide-Free Fluorescence In Situ Hybridization (FISH) |
|
|
135 | (6) |
|
|
|
|
|
141 | (6) |
|
|
|
|
|
|
|
Telomere Length Measurement by FISH |
|
|
147 | (6) |
|
|
|
|
|
|
|
|
|
RNA Imaging in Living Cells |
|
|
153 | (6) |
|
|
|
|
|
The Replicative Detargeting FISH (ReD-FISH) Technique in Studies of Telomere Replication |
|
|
159 | (12) |
|
|
|
|
|
Part III Material Suited for FISH Applications in Humans |
|
|
|
Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Bone Marrow, Chorion, Amniocytes, and Fibroblasts |
|
|
171 | (10) |
|
|
|
|
|
Application of FISH to Previously GTG-Banded and/or Embedded Cytogenetic Slides |
|
|
181 | (4) |
|
|
|
|
|
FISH in Uncultivated Amniocytes |
|
|
185 | (4) |
|
|
|
|
|
|
|
Tumorcytogenetic Diagnostics and Research on Blood and Bone Marrow Smears or Effusions |
|
|
189 | (6) |
|
|
|
|
|
|
|
|
|
Characterization of Mosaicism in Different Easy-to-Acquire Body Tissues Such As Buccal Smears, Skin Abrasions, Hair Root Cells, or Urine |
|
|
195 | (6) |
|
|
|
|
|
Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction |
|
|
201 | (8) |
|
|
|
FISH on Sperm, Spermatocytes and Oocytes |
|
|
209 | (18) |
|
|
|
Part IV Multicolor-FISH-Probe Sets (mFISH) and Immunostaining |
|
|
|
|
|
227 | (6) |
|
|
|
|
|
|
|
Multiplex FISH and Spectral Karyotyping |
|
|
233 | (8) |
|
|
|
|
|
|
|
241 | (8) |
|
|
|
|
|
|
|
|
|
249 | (8) |
|
|
|
|
|
|
|
Heterochromatin-Directed mFISH (HCM-FISH) |
|
|
257 | (4) |
|
|
|
|
|
|
|
|
|
Subtelomeric and/or Subcentromeric Probe Sets |
|
|
261 | (10) |
|
|
|
|
|
|
|
271 | (4) |
|
|
|
|
|
|
|
Fluorescence In Situ Hybridization onto DNA Fibres Generated Using Molecular Combing |
|
|
275 | (20) |
|
|
|
|
|
|
|
Parental Origin Determination FISH: Pod-FISH |
|
|
295 | (6) |
|
|
|
|
|
Simultaneous Fluorescence Immunostaining and FISH |
|
|
301 | (26) |
|
|
|
|
|
|
|
RNA-Directed FISH and Immunostaining |
|
|
327 | (10) |
|
|
|
|
|
Immunofluorescence Staining for Cytosine Modifications Like 5-Methylcytosine and Its Oxidative Derivatives and FISH |
|
|
337 | (10) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
CENP Antibodies Used Additionally to FISH |
|
|
347 | (8) |
|
|
|
|
|
|
|
|
Interphase FISH in Diagnostics |
|
|
355 | (6) |
|
|
|
|
|
Interphase FISH for Detection of Chromosomal Mosaicism |
|
|
361 | (12) |
|
|
|
|
|
|
|
|
|
373 | (6) |
|
|
|
|
|
|
|
379 | (6) |
|
|
|
|
|
|
|
Three-Dimensional Interphase Analysis Enabled by Suspension FISH |
|
|
385 | (10) |
|
|
|
|
|
Part VI Applications of FISH in Zoology, Botany and Microbiology |
|
|
|
Animal Probes and ZOO-FISH |
|
|
395 | (22) |
|
|
|
Alexander S. Graphodatsky |
|
|
Three-Dimensional Immunofluorescence In Situ Hybridization in Preimplantation Mouse Embryos |
|
|
417 | (12) |
|
|
|
|
|
Fish-FISH: Molecular Cytogenetics in Fish Species |
|
|
429 | (16) |
|
|
|
Luiz Antonio Carlos Bertollo |
|
|
|
|
FISH in Lampbrush Chromosomes |
|
|
445 | (14) |
|
|
|
|
|
General Protocol of FISH for Insects |
|
|
459 | (8) |
|
|
|
Luisa Antonia Campos Barros |
|
|
Silvia das Gracas Pompolo |
|
|
|
|
467 | (6) |
|
|
|
FISH on Insect Cells Transfected with Heterologous DNA |
|
|
473 | (4) |
|
|
|
In Situ Hybridization to Plant Chromosomes |
|
|
477 | (18) |
|
|
|
|
|
|
|
|
|
|
|
|
|
Yeast Chromosome Dynamics Revealed by Immuno FISH |
|
|
495 | (16) |
|
|
|
FISHing for Food Microorganisms |
|
|
511 | (20) |
|
|
|
|
|
|
|
|
|
|
|
Evaluation of Polymicrobial Involvement Using Fluorescence In Situ Hybridization (FISH) in Clinical Practice |
|
|
531 | (14) |
|
|
|
|
|
Gold-FISH: In Situ Hybridization of Microbial Cells for Combined Fluorescence and Scanning Electron Microscopy |
|
|
545 | (16) |
|
|
|
|
|
Part VII Comparative Genomic Hybridization (CGH) and Array CGH |
|
|
|
Comparative Genomic Hybridization (CGH) and Microdissection-Based CGH (Micro-CGH) |
|
|
561 | (6) |
|
|
|
|
|
|
|
|
|
567 | (20) |
|
|
|
|
|
Breakpoint Mapping of Balanced Chromosomal Rearrangements Using Array CGH of Microdissection-Derived FISH Probes |
|
|
587 | (10) |
|
|
|
|
|
Sequencing of Microdissection-Derived FISH Probes |
|
|
597 | (8) |
|
|
|
|
| Index |
|
605 | |
Lisainfo e-raamatute kohta