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E-raamat: Fluorescent Proteins: Methods and Protocols

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Fluorescent Proteins: Methods and ProtocolsSuurem pilt
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This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a wide range of biological systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Fluorescent Proteins: Methods and Protocols serves as an ideal guide for students and academicians enthusiastic about the recent progress in the practical application of fluorescent protein technology.
Using FPbase: The Fluorescent Protein Database.- Selecting the
Fluorescent Protein for In Vivo Imaging Experiments.- Advanced Fluorescence
Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo.-
Directed Evolution of Fluorescent Proteins in Bacteria.- Fluorescent
Proteins: Crystallization, Structural Determination, and Non-Natural Amino
Acid Incorporation.- Development and Characterization of Flavin-Binding
Fluorescent Proteins, Part I: Basic Characterization.- Development and
Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced
Characterization.- CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP11
Tag into the Genome of a Human Cell Line.- Fluorescent Protein-Based
Approaches for Sub-Cellular Protein Localization in Plants.- Growth Rates
Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying
Endogenously Expressed Fluorescent Protein Fusions.- DNA Visualization Using
Fluorescent Proteins.- Quantification ofIntracellular Citrate Concentrations
with Genetically Encoded Biosensors.- Quantification of Redox-Sensitive GFP
Cysteine Redox State via Gel-Based Read-Out.- Assessing In Vivo Oxygen
Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae.- Osmium
Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron
Microscopy of Epon-Embedded Mammalian Cultured Cells.- Fluorescence-Detection
Size-Exclusion Chromatography-Based Thermostability Assay for Membrane
Proteins.- Determining Protein-Protein Interaction with GFP-Trap Beads.
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