"Proteomics aims to study all the proteins of human and other living systems, and their properties to provide an integrated view of cellular processes. The proteomic study involves the application of high-throughput technologies that are rapidly evolvingand new platforms are coming forward regularly with versatile novel applications and are valuable tools for biomedical and pharmaceutical applications. This book provides a detailed understanding of the basics of proteins and proteomics, Gel based-proteomics techniques, basics of mass-spectrometry and quantitative proteomics, Interactomics: basics and applications, and advancement in proteomics. It also covers basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics. It also highlights the application and challenges of various high throughput integrated proteomics technologies capable of fast and accurate screening of thousands of biomolecules are found to be very effective for studying disease pathobiology and identification of next-generation biomarkers and potential drug/ vaccine targets, and therefore considered as valuable tools for multidisciplinary research. Features: Basics of proteins and proteomics techniques In-depth understanding of mass-spectrometry and quantitative proteomics An overview of interactomics and its application for translational research. Advancement in the field of proteomics and challenges in clinical applications. We hope the knowledge gained fromthe reading of the text will intrigue and motivate the young minds to explore future opportunities in the constantly evolving field of proteomics"--
This book provides a detailed understanding of the basics of proteins and proteomics, Gel based-proteomics techniques, basics of mass-spectrometry and quantitative proteomics, Interactomics: basics and applications, and advancement in proteomics.
| Preface |
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vii | |
| Author |
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ix | |
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Module I Basics of Proteins and Proteomics |
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1 Basics of Amino Acids and Proteins |
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3 | (10) |
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2 Protein Chemistry to Proteomics |
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13 | (12) |
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Module II Gel-Based Proteomics Techniques |
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25 | (20) |
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4 2D Difference Gel Electrophoresis (2D-DIGE) |
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45 | (10) |
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5 Gel-Based Proteomic Data Analysis |
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55 | (24) |
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Module III Basics of Mass Spectrometry and Quantitative Proteomics |
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6 Introduction to Mass Spectrometry |
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79 | (12) |
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7 Hybrid Mass Spectrometry Configurations |
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91 | (8) |
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8 Tandem Mass Spectrometry for Protein Identification |
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99 | (12) |
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9 In Vitro Quantitative Proteomics Using iTRAQ |
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111 | (12) |
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10 In Vivo Quantitative Proteomics Using SILAC |
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123 | (12) |
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Module IV Interactomics: Basics and Application |
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11 Introduction to Interactomics |
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135 | (10) |
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12 Antigen and Antibody Microarrays |
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145 | (16) |
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13 Cell-Free Expression-Based Protein Microarrays |
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161 | (16) |
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14 Nucleic Acid Programmable Protein Arrays |
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177 | (12) |
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189 | (16) |
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16 Surface Plasmon Resonance |
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205 | (10) |
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17 Surface Plasmon Resonance Imaging |
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215 | (6) |
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18 Protein Interaction Analysis Using SPR and SPRi |
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221 | (10) |
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Module V Advancements in Proteomics |
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19 Proteomics for Translational Research |
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231 | (12) |
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20 Future of Proteomics for Clinical Applications |
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243 | (8) |
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21 Challenges in Clinical Proteomics |
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251 | (8) |
| Index |
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259 | |
Prof. Sanjeeva Srivastava is a professor and the group leader of the Proteomics Laboratory at the Department of Biosciences and Bioengineering at the Indian Institute of Technology, Bombay. High-throughput proteomics, protein microarrays, and mass spectrometry are among his specialties. He has implemented ground-breaking AI-driven data analytics on big biological datasets. He has been at the forefront of biomedical research based on big data. His group's current research focuses on the development of clinical biomarkers for infectious diseases and malignancies. His group also pioneered therapeutic target identification efforts and decoded protein interaction networks in human illnesses like gliomas and contagious diseases like COVID-19 and malaria. His group has developed reliable diagnostic biomarkers and described the pathophysiology of severe malaria (falciparum and vivax) and COVID-19, especially the underlying mechanisms that lead to the development of severe sequelae.
Dr. Srivastava is an active contributor to global proteomics research and development. He serves on the Executive Committee of Human Proteome Organization (HUPO) and Proteomics Society, India (PSI). He has more than 125 publications from his work as an independent researcher at IIT Bombay. To date, he has filed 15 patents that include biomarkers for various types of cancers, infectious diseases like malaria, leptospirosis, COVID-19, and method improvement in uncharted territories such as fish and plant proteomics.
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