E-raamat: Galectins: Methods and Protocols

Galectins: An Ancient Family of Carbohydrate Binding Proteins with Modern Functions.- Cloning, Expression and Purification of Galectins for In Vitro Studies.- Purification of Recombinant Galectins Using Affinity Chromatography Methods.- Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation.- Rapid Detection and Purification of Galectin-3 by the Capture and Release (CaRe) Method.- Introducing 77Se NMR Spectroscopy to Analyzing Galectin-Ligand Interaction.- Evaluation of Galectin Binding by Surface Plasmon Resonance.- Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein.- Examining Galectin Binding Specificity Using Glycan Microarrays.- Mechanism of Mucin Recognition by Lectins - A Thermodynamic Study.- Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis.- Dissecting Context-Specific Galectin Binding Using Glyco-Engineered Cell Libraries.- Method for Identifying GalectinLigands on Lymphocyte Membrane Glycoproteins.- Transformation of Agrocybe Cylindracea Galectin into aGalNAc-specific Lectin.- What Happens if a Human Galectin Enters the Endoplasmic Reticulum?.- Investigation of Galectins in Frozen Tissue and Mammalian Cell Culture Using Confocal Microscopy.- Exploring the Galectin Network by Light and Fluorescence Microscopy.- Molecular Imaging for In Vivo Tracking and Detection of Galectin Binding Partners.- Visualization of Cytosolic Galectin Accumulation Around Damaged Vesicles and Organelles.- Transcytosis of Galectin-3 in Mouse Intestine.- Evaluating the Role of Galectins in Clathrin Independent Endocytosis.- Examination of Galectin-3-Recruitment into Multivesicular Bodies for Exosomal Secretion.- Manipulating Galectin Expression in Zebrafish (Danio Rerio).- Examining Galectin Gene Regulation by Reporter Assays.- Examining the Impact of Galectin-9 on Latent HIV Transcription.- Evaluation of the Role of Galectins in Parasite Immunity.- Evaluationof the Bactericidal Activity of Galectins.- Detection of Phosophatidylserine Exposure on Leukocytes Following Treatment with Human Galectins.- Detection of Reactive Oxygen Species in Human Neutrophils Under Various Conditions of Exposure to Galectin.- Analysis of Galectin-Binding Receptors on B Cells.- Methods for Assessing the Effects of Galectins on Leukocytetrafficking and Clearance.- Examination of the Contributions of Maternal/Placental-Derived Galectin-1 to Pregnancy Outcome.- Method to Study the Role of Galectins in Angiogenesis In Vivo Using the Chick Chorioallantoic Membrane Assay.- Untangling Galectin-Mediated Circuits that Control Hypoxia-Driven Angiogenesis.- Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology.- Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle.- Exploring the Role of Galectins in Cancer: In Vitro and In Vivo Approaches.