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E-raamat: Genotoxicity Assessment: Methods and Protocols

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Genotoxicity Assessment: Methods and ProtocolsSuurem pilt
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This current edition explores new tests for genotoxicity testing, along with other well-known techniques. This will further help in our understanding of the genotoxic effects of chemicals. The book has different sections dealing with various assays for gene mutation, chromosomal abnormalities, primary DNA damage, etc. It also delves into plant models, animals and their alternates, as well as in silico approaches for genetic toxicology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Genotoxicity Assessment: Methods and Protocols, Second Edition serves as a highly useful and ready resource for research students and scientists working in regulatory toxicology as well as biomedical, biochemical, and pharmaceutical sciences.
In Vitro Mouse Lymphoma Cell (L5178Y Tk+/- -3.7.2.C) Forward Mutation
Assay.- Erythrocyte-Based Pig-a Gene Mutation Assay.- Detection of In Vivo
Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes.- In Vitro
Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests.-
Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN)
Assay in the Field of Biological Dosimetry.- Chromosomal Aberration Test in
Human Lymphocytes.- In Vivo Micronucleus Assay in Mouse Bone Marrow.-
Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cells.-
Micronucleus Analysis by Flow Cytometry.- Hens Egg Test for Micronucleus
Induction (HET-MN).- Comparative Genomic Hybridization (CGH) in
Genotoxicology.- The Comet Assay: Assessment of In Vitro and In Vivo DNA
Damage.- The Comet Assay in Human Biomonitoring.- The Comet Assay in Marine
Animals.- Multicolour Laser Scanning Confocal Immunofluorescence Microscopy
of DNA Damage Response Biomarkers.- Detection of DNA Double Strand Breaks
Using Pulse Field Gel Electrophoresis.- Detection of UV-Induced Thymine
Dimers.- Micronucleus Assay with Tetrad Cells of Tradescantia.- The Wing-Spot
and the Comet Tests as Useful Assays for Detecting Genotoxicity in
Drosophila.- In Silico Approaches in Predictive Genetic Toxicology.
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