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E-raamat: Glaucoma: Methods and Protocols

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  • Sari: Methods in Molecular Biology 2858
  • Ilmumisaeg: 21-Oct-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071641408
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  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2858
  • Ilmumisaeg: 21-Oct-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071641408
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This second edition volume expands on the previous edition with an update on the latest methodologies used to study the neurodegeneration of glaucoma. This book notably features a focus on ocular hypertension as an important factor in the pathogenesis of glaucoma, and the relationship between elevated intraocular pressure and neurodegeneration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.





Cutting-edge and authoritative, Glaucoma: Methods and Protocols, Second Edition is a valuable resource for anyone interested in glaucoma research. This book will encourage innovation and facilitate progress toward improving our understanding and treatment of glaucoma.
Evaluation of Cross-Linked Actin Networks (CLANs) in Human Trabecular
Meshwork Cells and Tissues.- 3D Extracellular Matrix Protein Hydrogels for
Human Trabecular Meshwork Cell Studies.- Protocol for the Extraction and
Characterization of Trabecular Meshwork Cell Cytoskeletome
Fraction.- Measurements of Phagocytosis in Trabecular Meshwork
Cells.- Nanotubules and Cellular Communication in Trabecular Meshwork
Cells.- Isolation and Characterization of Exosomes from Ocular
Samples.- Timelapse Live-Cell Imaging Using Fluorescent Protein Sensors in
Outflow Pathway Cells Under Fluid Flow Conditions.- Human Anterior Segment
Perfusion Organ Culture.- Segmental Aqueous Humor Outflow.- Human Corneal Rim
Perfusion Culture.- The Inducible TGF2 Ocular Hypertension Mouse
Model.- Mouse Model of Glucocorticoid-Induced Glaucoma.- Trabecular Meshwork
Stem Cells for Glaucoma Treatment.- Aqueous Humor Angiography.- Retinal
Ganglion Cell Transplantation: Differentiation and Quantification
Strategies.- Retinal Ganglion Cell Subtypes and Their Vulnerability in
Glaucoma.- Full-Field Electroretinogram Responses in Rodent Models of
Ganglion Cell Injury.- Testing Visual Function by Assessment of the Optomotor
Reflex in Glaucoma.- Controlled Elevation of Intraocular Pressure (CEI)
Glaucoma Model in Rats.- A Comprehensive Protocol for Microbead-Induced
Ocular Hypertension in Mice.- Continuous Wireless Telemetric Measurement of
Intraocular Pressure (IOP), Ocular Perfusion Pressure (OPP), and
Cerebrospinal Fluid Pressure (CFP) in Nonhuman Primates (NHPs).- In Vitro and
In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve
Regeneration.