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E-raamat: Glycan Microarrays: Methods and Protocols

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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2460
  • Ilmumisaeg: 01-Jan-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071621486
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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2460
  • Ilmumisaeg: 01-Jan-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071621486
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This volume encompasses short targeted reviews and methods featuring the construction and use of glycan microarray platforms. Chapters guider readers through glycan microarrays comprised of synthetic glycans, natural biomolecules, bacteria, neoglycoproteins,  various applications and progress of these platforms. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Glycan Microarrays: Methods and Protocols aims to highlight the interdisciplinary nature of the development and use of the glycan microarray platform.

Neoglycoprotein and glycoprotein printing on a hydrogel functionalized
microarray surface and incubation with labeled lectins.- Evaluation of
glycan-binding specificity by glycoconjugate microarray with an
evanescent-field fluorescence detection system.- Multiplex glycan bead array
(MGBA) for high throughput and high content analyses of glycan binding
proteins including natural anti-glycan antibodies.- Nanocube-based fluidic
glycan array.- General strategies for glycan microarray data processing and
analysis.- Calculating half maximal inhibitory concentration (IC50) values
from glycomics microarray data using GraphPad Prism.- Synthetic plant glycan
microarrays as tools for plant biology.- Mucin purification and printing
natural mucin microarrays.- Bacterial microarrays for examining bacterial
glycosignatures and recognition by host lectins.- Tissue glycome mapping:
Lectin microarray-based differential glycomic analysis of formalin-fixed
paraffin-embedded tissue sections.- Detection of autoantibodies using
combinatorial glycolipid microarrays.- Glycan microarrays containing
synthetic Streptococcus pneumoniae CPS fragments and their application to
vaccine development.- Lectin-based protein microarray for the glycan analysis
of colorectal cancer biomarkers: the insulin-like growth factor
system.- Bacterial staining and profiling for glycan interactions on glycan
microarrays for t-test calculation.- Preparation and fluorescent labelling of
cell-derived micelles and profiling on glycan microarrays.