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E-raamat: Hematopoietic Stem Cell Protocols

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Updating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protocols from leading stem cell researchers. This in-depth volume presents a clear view of the landscape of assays available to the stem cell researcher working in the growing hematopoietic stem cell (HSC) field. A robust and active field, it is supported by an abundance of innovative mouse models and molecular tools for analysis of phenotypes and functions in mouse and human cells. Understanding more about hematopoietic stem cell biology is integral if these versatile cells are to be applied effectively to treat and cure a wide range of blood diseases. An introductory chapter puts the major contributions of the book into the proper perspective. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Third Edition is a much needed technical resource in the critically important field of hematopoietic stem cell investigation.

Preface v
Contributors ix
PART I OVERVIEW
1 The Hematopoietic Stem Cell Landscape
3(6)
Kevin D. Bunting
Cheng-Kui Qu
Part II Stem Cell Enrichment and Heterogeneity
2 Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow
9(12)
Huimin Cao
Brenda Williams
Susan K. Nilsson
3 Single-Cell PCR Profiling of Gene Expression in Hematopoiesis
21(22)
Jose Teles
Tariq Enver
Cristina Pina
4 Hematopoietic Stem and Progenitor Cell Mobilization in Mice
43(22)
Jonathan Hoggatt
Tiffany A. Tate
Louis M. Pelus
5 Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells
65(14)
Brahmananda Reddy Chitteti
Edward F. Srour
6 Flow Cytometric Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells
79(12)
Lei Dong
Cheng-Kui Qu
Part III Molecular and Cellular Characterization
7 Gene Expression Profiling of Hematopoietic Stem Cells (HSCs)
91(30)
Nalini Raghavachari
8 Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells
121(20)
Wenhuo Hu
Christopher Y. Park
9 DNA Methylation Profiling of Hematopoietic Stem Cells
141(14)
Amber Hogart Begtrup
10 Metabolic Characterization of Hematopoietic Stem Cells
155(10)
Fatih Kocabas
Junke Zheng
Chengcheng Zhang
Hesham A. Sadek
11 Nanoproteomic Assays on Hematopoietic Stem Cells
165(16)
Heath L. Bradley
Himalee Sabnis
Deborah Pritchett
Kevin D. Bunting
Part IV In Vitro Assays and Differentiation
12 Hematopoietic Differentiation of Pluripotent Stem Cells in Culture
181(14)
Jason A. Mills
Prasuna Paluru
Mitchell J. Weiss
Paul Gadue
Deborah L. French
13 Ex Vivo Assays to Study Self-Renewal, Long-Term Expansion, and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells
195(16)
Pallavi Sontakke
Marco Carretta
Marta Capala
Hein Schepers
Jan Jacob Schuringa
14 Ex Vivo Expansion of Murine and Human Hematopoietic Stem Cells
211(12)
Phuong L. Doan
John P. Chute
15 Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level
223(14)
Byungwook Ahn
Zhengqi Wang
David R. Archer
Wilbur A. Lam
Part V Transplantation Assays and Imaging Engraftment
16 Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells
237(10)
Ryo Yamamoto
Yohei Morita
Hiromitsu Nakauchi
17 Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull
247(20)
Juwell W. Wu
Judith M. Runnels
Charles P. Lin
18 Immunodeficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development
267(12)
Ken-Edwin Aryee
Leonard D. Shultz
Michael A. Brehm
19 Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells
279(8)
Xi C. He
Zhenrui Li
Rio Sugimura
Jason Ross
Meng Zhao
Linheng Li
Part VI Genetic Modification
20 Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells
287(24)
Marioara F. Ciuculescu
Christian Brendel
Chad E. Harris
David A. Williams
21 Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells
311(10)
Niek P. van Til
Gerard Wagemaker
22 High-Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies
321(24)
Brian C. Beard
Jennifer E. Adair
Grant D. Trobridge
Hans-Peter Kiem
23 Barcoded Vector Libraries and Retroviral or Lentiviral Barcoding of Hematopoietic Stem Cells
345(16)
Leonid V. Bystrykh
Gerald de Haan
Evgenia Verovskaya
Index 361