Preface |
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Contributors |
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1 The Hematopoietic Stem Cell Landscape |
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3 | (6) |
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Part II Stem Cell Enrichment and Heterogeneity |
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2 Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow |
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9 | (12) |
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3 Single-Cell PCR Profiling of Gene Expression in Hematopoiesis |
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21 | (22) |
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4 Hematopoietic Stem and Progenitor Cell Mobilization in Mice |
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43 | (22) |
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5 Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells |
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65 | (14) |
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Brahmananda Reddy Chitteti |
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6 Flow Cytometric Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells |
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79 | (12) |
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Part III Molecular and Cellular Characterization |
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7 Gene Expression Profiling of Hematopoietic Stem Cells (HSCs) |
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91 | (30) |
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8 Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells |
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121 | (20) |
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9 DNA Methylation Profiling of Hematopoietic Stem Cells |
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141 | (14) |
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10 Metabolic Characterization of Hematopoietic Stem Cells |
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155 | (10) |
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11 Nanoproteomic Assays on Hematopoietic Stem Cells |
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165 | (16) |
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Part IV In Vitro Assays and Differentiation |
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12 Hematopoietic Differentiation of Pluripotent Stem Cells in Culture |
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181 | (14) |
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13 Ex Vivo Assays to Study Self-Renewal, Long-Term Expansion, and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells |
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195 | (16) |
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14 Ex Vivo Expansion of Murine and Human Hematopoietic Stem Cells |
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211 | (12) |
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15 Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level |
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223 | (14) |
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Part V Transplantation Assays and Imaging Engraftment |
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16 Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells |
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237 | (10) |
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17 Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull |
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247 | (20) |
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18 Immunodeficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development |
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267 | (12) |
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19 Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells |
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279 | (8) |
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Part VI Genetic Modification |
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20 Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells |
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287 | (24) |
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21 Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells |
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311 | (10) |
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22 High-Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies |
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321 | (24) |
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23 Barcoded Vector Libraries and Retroviral or Lentiviral Barcoding of Hematopoietic Stem Cells |
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345 | (16) |
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Index |
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361 | |