Microscopic observations in histochemistry and cytochemistry became rare after the 1980s, and now that some researchers are using them again in specific cases, references on them are difficult to find. Researchers mostly in France, but also Belgium and Britain, explain the basics of using both light and transmission electron microscopy in the two fields, describing several classical techniques for both experienced and novice researchers, technicians, and students. Among the topics are enzyme histochemistry methods, visualizing apoptosis, cytochemical techniques, preparing samples for electron microscopy, and image quantification in histology and cytology. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com)
Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualization describes the essential techniques that can be used for histochemical investigations in both light and transmission electron microscopy.
The book begins by discussing techniques in light microscopy. It reviews classical methods of visualization, histochemical and histoenzymatic methods, and methods used to visualize cell proliferation and apoptosis. Next, the book examines the cytochemical methods used in electron microscopy with traditional techniques, as well as more specialized methods. The final section provides an overview of image analysis and describes how image processing methods can be used to extract vital information. A 16-page insert supplies color illustrations to enhance the text.
Techniques will continue to adapt to the latest technological innovations, allowing more and more precise quantification of images. These developments are essential to the biological as well as the medical sciences. This manual is a critical resource for novice and experienced researchers, technicians, and students who need to visualize what happens in the cell, the molecules expressed, the main enzymatic activities, and the repercussions of the molecular activities upon the structure of the cells in the body.
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