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E-raamat: HIV Protocols

  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2807
  • Ilmumisaeg: 14-May-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071638620
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  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2807
  • Ilmumisaeg: 14-May-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071638620
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This fourth edition volume expands on the previous editions with discussions on the latest methodologies to study HIV, live cell imaging, HIV cure, new modifications to the viral RNA that impacts HIV biology, and new types of intracellular compartments. The chapters in this book are organized into seven parts and cover topics such as HIV latency reactivation via single molecule RNA detection; T cell responses; new and efficacious anti-HIV CAR T cells; analysis of mucosal HIV infection; analysis of 3D brain organoids to study neuro AIDS; and the transfer of antibodies across the blood brain barrier. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, HIV Protocols, Fourth Edition is a valuable resource for all preclinical HIV-1 researchers looking to learn more about this important and advancing field. 

Infectious Virus Tracking by Fluorescent Live Cell Imaging in Primary
Cells.- Imaging HIV-1 Nuclear Import, Uncoating, and Proviral
Transcription.- RNA-FISH for HIV Transcription/Localization
Analysis.- Single-Cell and Single-Molecule RNA-FISH Combined with
Immunofluorescence and High-Speed and High-Resolution Scanning Analysis to
Visualize Reactivation of Latent HIV-1.- Visualizing HIV-1 Assembly at the T
Cell Plasma Membrane using Single Molecule Localization: TIRF
Microscopy.- Single-Virion Analysis: A Method to Visualize HIV-1 Particle
Content Using Fluorescence Microscopy.- Correlative Imaging to Detect Rare
HIV Reservoirs and Associated Damage in Tissues.- Characterization of Nuclear
HIV-Induced Membraneless Organelles through Fluorescence
Microscopy.- Detection of CPSF6 in Biomolecular Condensates as a Reporter of
Nuclear Import.- Monitoring HIV-1 Nuclear Import Kinetics Using a Chemically
Induced Nuclear Pore Blockade Assay.- Biochemical Detection of Capsid in the
Nucleus During HIV-1 Infection.- Chromatin Immunoprecipitation of Retroviral
Genomes with Antibodies Recognizing Modified Histones and Specific Viral
Proteins.- Measurement of HIV Rev-Rev Response Element Functional
Activity.- Method for the Enrichment of N6-Methyladenosine-Modified Cellular
and HIV-1 RNA.- Direct Analysis of HIV mRNA m6A Methylation by Nanopore
Sequencing.- Identifying Individual Pseudouridine () Sites Across
Transcripts from HIV-1 Infected Cells.- Proximity Ligation Assay to Detect
the Proximity between Host Proteins and Viral Proteins of HIV-1.- Modeling
HIV-1 Infection in CNS via Infected Monocytes using Immunocompetent Brain
Organoids.- An In Vitro Model of Blood Brain Barrier for Studies on HIV
Neuroinflammation and CNS Antibody Penetration.- Generation of Anti-HIV CAR-T
Cells for Preclinical Research.- Highly Sensitive Analysis of Cervical
Mucosal HIV-1 Infection using Reporter Viruses Expressing Secreted
Nanoluciferase.- Multiparameter Flow Cytometry Monitoring of T Cell
Responses.- An Ultrasensitive p24 Assay to Measure HIV-1 in Diverse
Biological Matrixes.